Nucleocytoplasmic-localized acyltransferases catalyze the malonylation of 7-O-glycosidic (iso)flavones in Medicago truncatula
Article first published online: 21 MAY 2008
No claim to original US government works. Journal compilation © 2008 Blackwell Publishing Ltd
The Plant Journal
Volume 55, Issue 3, pages 382–396, August 2008
How to Cite
Yu, X.-H., Chen, M.-H. and Liu, C.-J. (2008), Nucleocytoplasmic-localized acyltransferases catalyze the malonylation of 7-O-glycosidic (iso)flavones in Medicago truncatula. The Plant Journal, 55: 382–396. doi: 10.1111/j.1365-313X.2008.03509.x
- Issue published online: 23 JUL 2008
- Article first published online: 21 MAY 2008
- Received 15 February 2008; revised 17 March 2008; accepted 20 March 2008; published online 21 May 2008.
Figure S1. pH preferences of the intact MtMaT1 (a) and the truncated MtMaT1-&Dgr;110 (b).
Figure S2. HPLC profiles of phenolic extracts from individual transgenic lines of tt3/tt6-IFS-MtMaT1 (a) and tt3/tt6-IFS (b) that are different from those lines used in Figure 6a and b. The arrow points the novel malonylated genistein 7-O-glucoside produced by overexpression of MtMaT1. Note that peak (iv) in Figure 6a and several peaks in Figure 6b are varied in the individual plants (a and b). Panels in the middle and right columns showing UV spectra of the peaks labeled in Figure 6 (solid line), and the generally related reference spectra are shown with dotted line.
Figure S3 Isoflavone 7-O-methyltransferase fused with GFP and expressed in M. truncatula leaf epidermal cells under normal growth condition, fluorescence signal was not observed in nucleus.
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