Figure  S1. Structure of T-DNAs used to create the CCV lines in Arabidopsis is shown. (a) JM71, Ethanol-inducible EYFP-LacI-NLS fusion protein. RB, LB, right and left borders; EYFP-LacI-NLS; enhanced yellow florescent protein-lac repressor-nuclear localization signal; pAlcA,Aspergillus nidulans AlcA promoter; NPTII, neomycin phosphotransferase gene cassette; p35S, CaMV 35S promoter; AlcR, A.  nidulans ALCR transcription factor. (b) EL700S, Dex-inducible mGFP (Green fluorescent protein)-LacI-NLS. GVG, glucocorticoid-binding domain-VP16 acidic activation domain-Gal4 DNA-binding domain with pea rbcs-E9 terminator, HPT, hygromycin phosphotransferase with NOS promoter and terminator; 6xUAS, 6xGal4 UAS; TATA, TATA box; with pea rbcs-3A terminator.

Figure  S2. RLA in shoots after AZA treatment. Treatment with the DNA methylation inhibitor AZA did not alter the RLA in shoots for CCT lines on the short arm of Ch2. RLA is calculated by setting the observed activity from shoot tissue of our control line, CCP4-20, to 100. Data represents the mean ± SE from triplicate assay samples.

Figure  S3. Chromatin dynamics are altered for roots, but not leaves, treated with AZA. Overall mean squared differences in distances < Dd2 > (y-axis, μm2) between LacO spots plotted against the time interval, Dt (x-axis, min) for CCP4.211 root (a), CCP4.211, CCT431, CCT396, and CCT432 leaf (b–e). Lines are black (MS roots), blue (AZA roots), gray (MS leaf) or green (AZA leaf) to indicate treatment and tissue. Data sets represent the mean ± SE of n pairs of distances for each line. Following AZA treatment, CCP4.211 root endoreduplicated nuclei undergo changes in chromatin dynamics with an increased diffusion coefficient and radius of confinement. Treatment with AZA did not greatly affect the confinement radius or diffusion coefficient in leaf nuclei for CCP4.211, CCT431, and CCT396; however, AZA leaves of CCT432 exhibited greater mobility compared to MS leaves.

Table  S1. Primers used for locus-specific PCR and ABRC stock numbers are listed for the CCP4, CCP5 and CCT lines.

Table  S2. Chromosomal position, relative luciferase activity (RLA) and overall level of GUS activity is given for each line in the collection.

Appendix  S1. Protocol: more details of the construction of the CCP4 cassette.

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