SEARCH

SEARCH BY CITATION

Figure  S1. Heating device. Homemade massive aluminium container manufactured to cover the bottom part of a conventional plastic exsiccator used for vacuum infiltration of paraffin into plant material at elevated temperatures. After heating (e.g. in a drying cabinet), of the bottom part, the temperature was kept constant for 30–60 min.

Figure  S2. Cooling device. Filling of the microtome attachment with dry ice kept the complete cutting unit cool, leading to superior cutting conditions (a) Microtome without and (b) with homemade cooling device. The slots are filled with dry ice during usage.

Figure  S3. Metabolite and mineral profile of Arabidopsis phloem exudate. (a) Content of sucrose (Suc), glucose (Glu), fructose (Fru) and mannitol (Man) and (b) of potassium (K), calcium (Ca), phosphorus (P), sulphur (S) and magnesia (Mg) (c) Detectable amounts of proteinaceous amino acids and other N-compounds.

Figure  S4. Test for transcripts of damaged cells contaminating the phloem exudates.

Lanes 1, 2, 3, 5, 7, and 9 show RT-PCR products of phloem exudate cDNA samples and lanes 4, 6, or 8 PCR-products of genomic DNA as control for PCR conditions. Note, that transcripts of CUT, a marker of epidermal cells, and CA, of mesophyll cells, could not be amplified in phloem exudates but in genomic DNA (lanes 6 and 8), whereas the phloem-specific CCH was found in exudates, CACT, actin2/8-cDNA; gACT, actin2/8 genomic DNA; cCA, chloroplastic anhydrase-cDNA (At3g01500); gCA, chloroplastic anhydrase genomic DNA; cCUT1, CUTIN1-cDNA (At1g68530); gCUT1, CUTIN1 genomic DNA cCCH, copper chaperone-cDNA; gCCH, CCH genomic DNA; M, 100 bp ladder.

Figure  S5. Distribution of phloem transcripts in functional categories according to MapMan Version 2.0.0, September 2006; http://gabi.rzpd.de/projects/MapMan/download/; Usadel et al., 2005. (a) 2417 transcripts of the phloem exudate samples, (b) 1291 transcripts of the laser microdiction-derived phloem tissue samples and (c) 444 companion cell expressed sequence tags. Not shown is the group of ‘not assigned’ with unknown function which comprises for exudate 651 and LMPC-derived phloem tissue 436 transcripts.

Figure S6. Nutrient depletion-induced genes.

Figure S7. Phytohormone-induced genes.

Figure S8. Light-induced genes.

Figure S9. Genes activated by all three light qualities.

Table  S1. Transcripts of Arabidopsis phloem exudate, companion cells or LMPC-derived phloem sections. The first 3 sub-tables ‘1) exudate’, ‘2) LMPC’, and ‘3) CC-ESTs’ list the transcripts present in the Arabidopsis phloem exudate, phloem tissue (isolated by laser microdissection pressure catapulting, LMPC) or in the companion cell-expressed-sequence-tag-library (CC-EST;s Ivashikina et al., 2003). ‘Sub-table 4)’ comparison graphically shows a comparison of the 2417 exudate and 1291 phloem tissue transcripts with those of the CC- EST-library. Sub-table 5) intersection_114 lists the 114 transcripts which are present in the three phloem data sets. Individual genes of the sub-tables 1) to 3) and 5) were sorted into functional categories according to MapMan (http://gabi.rzpd.de/projects/MapMan/). Each sub-table shows the following columns: Number of the MapMan pathway (BINS), metabolic pathway (Pathway), Arabidopsis gene index (AGI) and putative function (Description). Grey colour markes duplicate genes of different pathways according to MapMan.

Table  S2. Comparison of phloem annotated genes with transcripts of the phloem exudate, LMPC-derived phloem sections and companion cells. The sub-table ‘Annotated genes_phloem or xylem’ lists fourteen respectively two transcripts detected in the data sets of phloem exudate-, phloem tissue (isolated by laser microdissection pressure catapulting; LMPC), or companion cell-expressed-sequence-tag-library (CC-ESTs; Ivashikina et al., 2003) according to a search in the database TAIR (www.Arabidopsis.org, March 2006). The following keywords have been used for the search: ‘phloem’, ‘sieve tube member’, ‘companion-cell’ and ‘xylem’.

Table  S3. Phloem exudate transcripts induced by nutrient-depletion. Sub-tables (1, 3, 5) with the ending ‘intersection’ graphically show the number of exudate transcripts which out of 2417 overlap with the number of genes induced by potassium (K), nitrogen (NO3), or sulphur (SO4) according to data sets from Genvestigator V3 (https://www.genevestigator.ethz.ch/). Sub-tables (2, 4, 6) with the ending ‘list’ contain exudate transcripts which are at least 3-fold-induced by depletion of either stimulus. Sub-tables are presented in the following order: 1) low K-induced_intersection, 2) low K-induced_list, 3) low NO3-induced_intersection, 4) low NO3-induced_list, 5) SO4-depletion_intersection, 6) SO4-depletion_list. Individual genes were sorted into functional categories according to MapMan (http://gabi.rzpd.de/projects/MapMan/). Each sub-table shows the following columns: Number of the MapMan pathway (BINS), metabolic pathway (Pathway), Arabidopsis gene index (AGI) and putative function (Description). Grey colour marks duplicate genes of different pathways according to MapMan.

Table  S4. Phloem exudate transcripts induced by hormone treatment. Sub-tables (1, 3, 5) with the ending ‘intersection’ graphically show the number of exudate transcripts which out of 2417 overlap with the number of genes induced by either gibberellic acid (GA3), indole acetic acid (IAA), or cytokinin (zeatin) according to data sets from Genvestigator V3 (https://www.genevestigator.ethz.ch/). Sub-tables (2, 4, 6) with the ending ‘list’ contain the exudate transcripts which are at least 3-fold-induced by the respective hormone. Sub-tables are presented in the following order: 1) GA3-induced_intersection, 2) GA3-induced_list, 3) IAA-induced_intersection, 4) IAA-induced_list, 5) zeatin-induced_intersection, 6) zeatin-induced_list. Individual genes were sorted into functional categories according to MapMan (http://gabi.rzpd.de/projects/MapMan/). Each sub-table shows the following columns: Number of the MapMan pathway (BINS), metabolic pathway (Pathway), Arabidopsis gene index (AGI) and putative function (Description). Grey colour marks duplicate genes of different pathways according to MapMan.

Table  S5. Phloem exudate transcripts induced by different light qualities. Sub-tables (1, 3, 5) with the ending ‘intersection’ graphically show the number of exudate transcripts which out of 2417 build an intersection with the number of genes induced by either blue light (BL), red light (RL), or far red light (FRL) according to data sets from Genvestigator V3 (https://www.genevestigator.ethz.ch/). Sub-tables (2, 4, 6) with the ending ‘list’ contain the exudate transcripts which are at least 3-fold-induced by the respective light quality. Sub-tables 7) and 8) compare the intersections of the BL, RL, FRL-dependent exudate transcripts to identify those genes which overlap with all three light qualities and sub-tables 9) to 11) show those which are specific for either of the light qualities. Sub-tables are presented in the following order: 1) BL-induced_intersection, 2) BL-induced_list, 3) RL-induced_intersection, 4) RL-induced_list, 5) FRL-induced_intersection, 6) FRL-induced_list, 7) BL_RL_FRL_exudate_intersect, 8) BL_RL_FRL_exudate_list, 9) 3_BL-specific_list, 10) 33_RL-specific_list, 11) 10_FRL-specific_list. Individual genes were sorted into functional categories according to MapMan (http://gabi.rzpd.de/projects/MapMan/). Each sub-table shows the following columns: Number of the MapMan pathway (BINS), metabolic pathway (Pathway), Arabidopsis gene index (AGI) and putative function (Description). Grey colour marks duplicate genes of different pathways according to MapMan.

Figure/Table  S6. Nutrient-depletion induced genes. Arabidopsis phloem exudate transcripts induced by potassium starvation (29 genes), low nitrate (12 genes), or sulphur depletion (3 genes) according to a biomarker search in Genevestigator (https://www.genevestigator.ethz.ch/).

Figure/Table  S7. Phytohormone-induced genes. Arabidopsis phloem exudate transcripts induced by zeatin (5 genes), giberellic acid (2 genes), or indole acetic acid (3 genes) according to a biomarker search in Genevestigator (https://www.genevestigator.ethz.ch/).

Figure/Table  S8. Light-induced genes. Arabidopsis phloem exudate transcripts induced by blue light (41 genes), red light (33 genes), or far red light (48 genes) according to a biomarker search in genevestigator (https://www.genevestigator.ethz.ch/).

Figure/Table  S9. Genes activated by all three light qualities. Thirty one phloem exudate transcripts of the light induced genes shown in Figure/Table S8 are activated by all three light regimes and only three by blue, one by red and nine by far red light alone.

Please note: Blackwell Publishing are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
TPJ_3555_sm_figs.pdf788KSupporting info item
TPJ_3555_sm_table1.xls167KSupporting info item
TPJ_3555_sm_table2.xls1226KSupporting info item
TPJ_3555_sm_table3.xls88KSupporting info item
TPJ_3555_sm_table4.xls80KSupporting info item
TPJ_3555_sm_table5.xls144KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.