Arabidopsis XXT5 gene encodes a putative α-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis

Authors

  • Olga A. Zabotina,

    1. Institute for Integrative Genome Biology, Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
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    • These authors contributed equally.

    • Present address: Department of Biochemistry, Biophysics and Mol. Biology, Iowa State University, 1210 Molecular Biology building, Ames, IA 50011, USA.

  • Wilhelmina T.G. Van De Ven,

    1. Institute for Integrative Genome Biology, Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
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    • These authors contributed equally.

  • Glenn Freshour,

    1. Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602, USA
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  • Georgia Drakakaki,

    1. Institute for Integrative Genome Biology, Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
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  • David Cavalier,

    1. Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
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  • Gregory Mouille,

    1. Laboratoire de Biologie Cellulaire, INRA, Route de Saint Cyr, 78026 Versailles Cedex, France
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  • Michael G. Hahn,

    1. Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602, USA
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  • Kenneth Keegstra,

    1. Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
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  • Natasha V. Raikhel

    Corresponding author
    1. Institute for Integrative Genome Biology, Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
      *(fax +(951)8272155; e-mail natasha.raikhel@ucr.edu).
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Errata

This article is corrected by:

  1. Errata: Correction Volume 57, Issue 2, 386, Article first published online: 13 January 2009

  • 1

    The naming of these genes has been changed recently (Cavalier et al., in press) to more clearly reflect that both genes encode xyloglucan xylosyltransferases and to better conform to the gene nomenclature standards adopted by the Arabidopsis community (Meinke and Koornneef, 1997). Thus, AtXT1 has become XXT1 and AtXT2 has become XXT2.

*(fax +(951)8272155; e-mail natasha.raikhel@ucr.edu).

Summary

The function of a putative xyloglucan xylosyltransferase from Arabidopsis thaliana (At1g74380; XXT5) was studied. The XXT5 gene is expressed in all plant tissues, with higher levels of expression in roots, stems and cauline leaves. A T-DNA insertion in the XXT5 gene generates a readily visible root hair phenotype (root hairs are shorter and form bubble-like extrusions at the tip), and also causes the alteration of the main root cellular morphology. Biochemical characterization of cell wall polysaccharides isolated from xxt5 mutant seedlings demonstrated decreased xyloglucan quantity and reduced glucan backbone substitution with xylosyl residues. Immunohistochemical analyses of xxt5 plants revealed a selective decrease in some xyloglucan epitopes, whereas the distribution patterns of epitopes characteristic for other cell wall polysaccharides remained undisturbed. Transformation of xxt5 plants with a 35S::HA-XXT5 construct resulted in complementation of the morphological, biochemical and immunological phenotypes, restoring xyloglucan content and composition to wild-type levels. These data provide evidence that XXT5 is a xyloglucan α-1,6-xylosyltransferase, and functions in the biosynthesis of xyloglucan.

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