SEARCH

SEARCH BY CITATION

Figure  S1. MS/MS analysis of pAtPSK4-myc. (a) Amino acid sequence of ppAtPSK4-myc is shown in which the signal peptide is underlined, peptide sequences identified by MALDI-TOF are highlighted in red and corresponding mass peaks are indicated. (b) MS/MS spectrum of peptide with mass peak of 974.5 derived from the myc tag. (c) MS/MS spectrum of peptide with mass peak of 576.3 derived from the precursor region of ppAtPSK4-myc. (d) MS/MS spectrum of peptide with mass peak of 690.4 derived from the precursor region of ppAtPSK4-myc.

Figure  S2. RT-PCR analysis of AtSBT1.1 transcripts in wt and sbt1.1-1 and -2 mutants. RNA was obtained from 14-day-old seedlings.

Figure  S3. pAtPSK4-myc processing is restored in F1 hybrids resulting from an outcross of 35S:ppAtPSK4-myc sbt1.1-1 to wild type. Western blot of extracts from root explants at 0 time or incubated for 1 day on CIM. 35S:ppAtPSK4-myc is expressed in a sbt1.1-1 background or in the F1 of a cross between sbt1.1-1 x wt.

Figure  S4. AtSBT1.1-YFP and AtPSK4-YFP are located in the apoplast. Plasmolysis of root cells from transgenic seedlings expressing AtSBT1.1-YFP (a–c) and AtPSK4-YFP (d–f) was carried out by treating root segments with 1 m KNO3 for 10 min. DIC images (a, d) were superimposed on epifluorescent images (b, e) to generate merged images (c, f). Bar = 50 μm.

Figure  S5. MS/MS spectrum for the cleaved N-terminal fluorogenic peptide (mass = 862.2). The parent peptide corresponds to residues RRSLVL of which each residue is represented by its b and y ions in the spectrum.

Table  S1. Primers used for PCR and qRT-PCR analysis.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
TPJ_3598_sm_figs S1-S5.pdf3353KSupporting info item
TPJ_3598_sm_table1.doc39KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.