Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography

Authors

  • Eli Kaminuma,

    1. Bioinformatics and Systems Engineering Division, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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  • Takeshi Yoshizumi,

    1. Plant Function Genomics Research Team, Plant Functional Genomics Research Group, RIKEN Yokohama Institute, Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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  • Takuji Wada,

    1. Gene Expression Research Team, Gene Discovery Research Group, RIKEN Yokohama Institute, Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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  • Minami Matsui,

    1. Plant Function Genomics Research Team, Plant Functional Genomics Research Group, RIKEN Yokohama Institute, Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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  • Tetsuro Toyoda

    Corresponding author
    1. Bioinformatics and Systems Engineering Division, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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Errata

This article is corrected by:

  1. Errata: Correction Volume 57, Issue 2, 387, Article first published online: 13 January 2009

(fax +81 45 503 9553; e-mail toyoda@base.riken.jp).

Summary

Quantitative morphological traits may be defined based on the 3D anatomy reconstructed from micro X-ray computed tomography (μCT) images. In this study, the heterogeneous spatial distribution of trichomes (hairs) on the adaxial leaf blade surface in Arabidopsis was evaluated in terms of 3D quantitative traits, including trichome number, average nearest-neighbour distance between trichomes, and proportion of large trichomes. The data reflect spatial heterogeneity in the radial direction, in that a greater number of trichomes were observed on the leaf blade margins relative to the non-margins, a distribution effect caused by the CAPRICE (CPC) and GLABRA3 (GL3) genes, which have previously been shown to affect trichome density. We further determined that the proportion of large trichomes on the blade mid-rib increases from the proximal end to the distal leaf tip in both wild-type plants and GL3 mutants. Our results indicate that the GL3 gene affects trichome distribution, rather than trichome growth, causing trichome initiation at the proximal base rather than the distal tip. On the other hand, CPC does affect trichome growth and developmental progression. Hence, quantitative phenotyping based on μCT enables precise phenotypic description for elucidation of gene control in morphological mutants.

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