These authors contributed equally to this work.
CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts
Version of Record online: 26 AUG 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
The Plant Journal
Volume 56, Issue 4, pages 590–602, November 2008
How to Cite
Chateigner-Boutin, A.-L., Ramos-Vega, M., Guevara-García, A., Andrés, C., De La Luz Gutiérrez-Nava, M., Cantero, A., Delannoy, E., Jiménez, L. F., Lurin, C., Small, I. and León, P. (2008), CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts. The Plant Journal, 56: 590–602. doi: 10.1111/j.1365-313X.2008.03634.x
- Issue online: 11 NOV 2008
- Version of Record online: 26 AUG 2008
- Received 26 February 2008; revised 19 June 2008; accepted 26 June 2008; published online 26 August 2008.
Figure S1.Comparison of the predicted PPR motifs of the CLB19 protein against the consensus sequences defined in Lurin et al. (2004).
Figure S2. Protein analysis of chloroplast photosynthetic proteins in Wt and in clb19 mutant. Total protein extracts were obtained from 15 d-old seedlings grown in low light conditions. Immunoblots were performed using antibodies against to the subunit B of ATP synthase (AtpB), the large subunit of RuBISCo (RbcL), a subunit of the cytochrome b6f complex (PetA), a subunit of photosystem I (PsaD) and a subunit of the cytocrome b/f (PetD). (a) 10 μg of total protein extract of wild-type (Wt) or clb19 were used in each lane. (b) For a more quantitative estimation of the difference in the AtpB levels, increasing amounts of total protein extract as indicated in each lane (μg) from Wt and clb19 were used. The Coomasie-stained gels (Coo.) are shown as a loading controls.
Figure S3. Alignment of RpoA and ClpP sequences. The amino acid sequences predicted by translation of representative bacterial or plastid rpoA and clpP genes were aligned using CLC Free Workbench 3.1 software (http//:www.clcbio.com). The amino acids altered by editing corroborated in Arabidopsis and Phalaenopsis (Zeng et al., 2007, Plant Cell Physiol48: 362) are indicated by an arrow. Editing restores amino acid conservation among plants for RpoA and with bacterial sequences in the case of ClpP.
Table S1. Comparison of chloroplastic transcripts abundance for clb19 and ptac2 mutants. Transcript abundance of all protein-encoding and ribosomal genes of the chloroplastic genome from the mutants (clb19-1, clb19-3 and ptac2) and wild type was measured by quantitative RT-PCR. The genes are listed in the same order as from right to left in Figure 4(b). The standard deviation was calculated from 3 technical replicates. The primers used are given in Supplementary Table 2.
Table S2. Primers used for genotyping plant material, preparing probes and for RACE and RNA editing analyses. All primers are given by the name used in the manuscript.
Table S3. Primers used for the quantitative RT-PCR experiments described in Figure 4(b) and in Supplementary Table 1.
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.
|TPJ_3634_sm_figS1.jpg||494K||Supporting info item|
|TPJ_3634_sm_figS2.jpg||155K||Supporting info item|
|TPJ_3634_sm_figS3.jpg||558K||Supporting info item|
|TPJ_3634_sm_tableS1.xls||30K||Supporting info item|
|TPJ_3634_sm_tableS2.doc||31K||Supporting info item|
|TPJ_3634_sm_tableS3.doc||36K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.