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Figure S1. Constructs used in this study.

Figure S2. Pairs of GFP/mRFP-AtVSR fusions localized tothe same PVC populations in protoplasts of tobacco BY-2 cells. When various pairs of GFP-AtVSR (green) or mRFP-AtVSR (red) fusions weretransiently co-expressed together as indicated in protoplasts of tobacco BY-2cells, they were all colocalized in the same PVCs. DIC, differentialinterference contrast. Scale bar = 50 μm.

Figure S3. Pairs of GFP/mRFP-AtVSR fusions localized tothe same PVC populations in protoplasts of Arabidopsis cells.

When various pairs of GFP-AtVSR (green) or mRFP-AtVSR (red) fusionswere transiently co-expressed together as indicated in protoplasts of Arabidopsiscells, they were all colocalized in the same PVCs. DIC, differential interference contrast. Scale bar = 50 μm.

Figure S4. Vacuolar transport of aleurain-GFP and 2S albumin-GFPin Arabidopsis protoplasts was mediated by the same PVCs. Aleurain-GFP (a) and 2S albumin-GFP (b) proteins were destined to vacuolesvia punctate PVC organelles. Both fluorescent fusion proteins showed punctatesignals at 6–12 h (early stage) after transformation, and diffused patternsinside the vacuoles occurred at 18–24 h (late stage) after transformation.DIC, differential interference contrast. Scale bar = 50 μm.

Figure S5. Aleurain-GFP and 2S albumin-GFP werecolocalized with the PVC marker mRFP-AtVSR2 in tobacco BY-2 protoplasts. Aleurain-GFP (a) and 2S albumin-GFP (b) proteins were colocalized withmRFP-AtVSR2 reporter as punctuated signals in electroporated protoplasts oftobacco BY-2 cells. DIC, differential interference contrast. Scale bar = 50 μm.

Figure S6. PVC organelles marked by AtVSR and Rab5homologs were distinct from the SCAMP1-positive TGN/EE in tobacco BY-2protoplasts. (a) YFP-SCAMP (green) signals were separated from the mRFP-Ara7 reporter(red) in tobacco BY-2 cells.

(b) YFP-SCAMP (green) signals were separated from the PVCmarker mRFP-AtVSR2 (red) in protoplasts of Arabidopsis suspensioncultures. DIC, differential interference contrast. Scale bar = 50 μm.

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TPJ_3645_sm_figS1.pdf125KSupporting info item
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TPJ_3645_sm_figS3.tif19211KSupporting info item
TPJ_3645_sm_figS4.tif8314KSupporting info item
TPJ_3645_sm_figS5.tif8243KSupporting info item
TPJ_3645_sm_figS6.tif8065KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.