A mutation in THERMOSENSITIVE MALE STERILE 1, encoding a heat shock protein with DnaJ and PDI domains, leads to thermosensitive gametophytic male sterility in Arabidopsis
Article first published online: 9 DEC 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
The Plant Journal
Volume 57, Issue 5, pages 870–882, March 2009
How to Cite
Yang, K.-Z., Xia, C., Liu, X.-L., Dou, X.-Y., Wang, W., Chen, L.-Q., Zhang, X.-Q., Xie, L.-F., He, L., Ma, X. and Ye, D. (2009), A mutation in THERMOSENSITIVE MALE STERILE 1, encoding a heat shock protein with DnaJ and PDI domains, leads to thermosensitive gametophytic male sterility in Arabidopsis. The Plant Journal, 57: 870–882. doi: 10.1111/j.1365-313X.2008.03732.x
- Issue published online: 19 FEB 2009
- Article first published online: 9 DEC 2008
- Received 14 June 2008; revised 13 September 2008; accepted 20 October 2008; published online 9 December 2008.
- THERMOSENSITIVE MALE STERILE 1;
- heat shock protein;
- male sterility;
In most flowering plant species, pollination and fertilization occur during the hot summer, so plants must have evolved a mechanism that ensures normal growth of their pollen tubes at high temperatures. Despite its importance to plant reproduction, little is known about the molecular basis of thermotolerance in pollen tubes. Here we report the identification and characterization of a novel Arabidopsis gene, THERMOSENSITIVE MALE STERILE 1 (TMS1), which plays an important role in thermotolerance of pollen tubes. TMS1 encodes a Hsp40-homologous protein with a DnaJ domain and an a_ERdj5_C domain found in protein disulfide isomerases (PDI). Purified TMS1 expressed in Escherichia coli (BL21 DE3) had the reductive activity of PDI. TMS1 was expressed in pollen grains, pollen tubes and other vegetative tissues, including leaves, stems and roots. Heat shock treatment at 37°C increased its expression levels in growing pollen tubes as well as in vegetative tissues. A knockout mutation in TMS1 grown at 30°C had greatly retarded pollen tube growth in the transmitting tract, resulting in a significant reduction in male fertility. Our study suggests that TMS1 is required for thermotolerance of pollen tubes in Arabidopsis, possibly by functioning as a co-molecular chaperone.