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Figure S1. The polar expression patterns of HD-ZIPIII genes are independent of ATS activity. (a), (b), (e), (f) and (i) wild-type Ler ovules; (c), (d), (g), (h) and (j) ats-1 mutant ovules; (a)–(d): in situ hybridization with antisense PHB probe. (e)–(h) in situ hybridization with antisense CNA probe. (i) and (j) in situ hybridization with antisense PHV probe.

Figure S2. Siliques of ats phb phv rev/+ plants have reduced seed set. (a) mature Ler silique. (b) mature phb phv rev/+ silique. (c) mature ats silique. (d) mature ats phb phv silique. (e) mature ats phb phv rev/+ silique. (f) seed set per silique; unique letters represent statistically different values. Scale bar = 0.1 cm.

Figure S3.PHABULOSA promoter activity measured by GUS staining in phb-6 ovules. (a) during integument initiation, PHB promoter is active in the chalaza and funiculus. (b) PHB promoter is active in both integuments. Abbreviations: nucellus (n), inner integument (ii), outer integument (oi).

Figure S4. Mutant seedling phenotypes observed in segregating F2 progeny from a selfed ats/+ cna/+ rev/+ phb phv plant. (a) and (b) seedlings with two cotyledons. (c) seedling with a single cotyledon. Scale bar = 0.1 cm.

Table S1. Oligonucleotides used in this study for genotyping. Names, sequence, corresponding allele and reference (if applicable) are given for each primer.

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