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Figure S1. Most of the GA-metabolism genes are expressed in pistils at anthesis. Expression was determined for genes of active GA biosynthetic enzymes GA 20-oxidase (light grey), GA 3-oxidase (medium grey), and deactivation enzymes GA 2-oxidase (dark grey). Pistils of cer6-2 flowers were harvested 2 h after dawn, corresponding with the first time-point of our experiments, depicted in Figure 6, 7 and 8 and Supplemental Figures 2 to 7 online. Each sample corresponded to a pool of at least 50 pistils. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized first to the expression of PP2A (At1g13320) and then to the expression of AtGA20ox1, which was used as reference level (expression level set to one). No expression was detected for AtGA3ox3 in these samples. Data correspond to mean and standard deviation (SD) of three technical replicates. Two independent experiments were carried out, with similar results.

Figure S2. Expression of GA 20-oxidase genes in fruits of cer6-2 at 2, 6, 24 and 48 h after treatment with mock (M), GA3 (G), NPA (N), or 2,4-D (D), as shown in Figure 8a. Time-course expression profile (2, 6, 24, and 48 h after treatment) is shown for AtGA20ox1, AtGA20ox2, AtGA20ox3, AtGA20ox4, and AtGA20ox5. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of PP2A (At1g13320) and then to the expression of mock-treated pistils at the 2 h time-point, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Figure S3. Expression of GA 3-oxidase genes in fruits of cer6-2 at 2, 6, 24 and 48 h after treatment with mock (M), GA3 (G), NPA (N), or 2,4-D (D), as shown in Figure 8a. Time-course expression profile (2, 6, 24, and 48 h after treatment) is shown for AtGA3ox1, AtGA3ox2, and AtGA3ox4. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of PP2A (At1g13320) and then to the expression of mock-treated pistils at the 2 h time-point, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Figure S4. Expression of GA 2-oxidase genes in fruits of cer6-2 at 2, 6, 24 and 48 h after treatment with mock (M), GA3 (G), NPA (N), or 2,4-D (D), as shown in Figure 8a. Time-course expression profile (2, 6, 24, and 48 h after treatment) is shown for AtGA2ox1, AtGA2ox2, AtGA2ox3, AtGA2ox4, AtGA2ox6, AtGA2ox7, and AtGA20ox8. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of PP2A (At1g13320) and then to the expression of mock-treated pistils at the 2 h time-point, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Figure S5. Expression of GA 20-oxidase genes in whole pistils (P), ovules (O) or valves (V) of cer6-2 at 6 h after treatment with mock, GA3, NPA, or 2,4-D, as shown in Figure 8b. Expression profile is shown for AtGA20ox1, AtGA20ox2, AtGA20ox3, AtGA20ox4, and AtGA20ox5. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of ACT8 (At1g49240) and then to the expression of the whole mock-treated pistils, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Figure S6. Expression of GA 3-oxidase genes in whole pistils (P), ovules (O) or valves (V) of cer6-2 at 6 h after treatment with mock, 330 μm GA3, 50 μm NPA, or 10 μm 2,4-D, as shown in Figure 8b. Expression profile is shown for AtGA3ox1, AtGA3ox2, and AtGA3ox4. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of ACT8 (At1g49240) and then to the expression of the whole mock-treated pistils, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Figure S7. Expression of GA 2-oxidase genes in whole pistils (P), ovules (O) or valves (V) of cer6-2 at 6 h after treatment with mock, 330 μm GA3, 50 μm NPA, or 10 μm 2,4-D, as shown in Figure 8b. Expression profile is shown for AtGA2ox1, AtGA2ox2, AtGA2ox3, AtGA2ox4, AtGA2ox6, AtGA2ox7, and AtGA20ox8. Each sample corresponded to a pool of at least 50 pistils/fruits. Expression levels were determined by qRT-PCR according to Materials and Methods. Data were normalized to the expression of ACT8 (At1g49240) and then to the expression of the whole mock-treated pistils, which was used as reference level (expression level set to one). Data correspond to mean and SD of three technical replicates.

Table S1. Genes of GA metabolism and oligos used for qRT-PCR.

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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.