These authors contributed equally.
Natural variation in maize architecture is mediated by allelic differences at the PINOID co-ortholog barren inflorescence2
Article first published online: 23 FEB 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 58, Issue 4, pages 618–628, May 2009
How to Cite
Pressoir, G., Brown, P. J., Zhu, W., Upadyayula, N., Rocheford, T., Buckler, E. S. and Kresovich, S. (2009), Natural variation in maize architecture is mediated by allelic differences at the PINOID co-ortholog barren inflorescence2. The Plant Journal, 58: 618–628. doi: 10.1111/j.1365-313X.2009.03802.x
- Issue published online: 14 MAY 2009
- Article first published online: 23 FEB 2009
- Received 14 November 2008; revised 29 December 2008; accepted 9 January 2009; published online 23 February 2009.
- barren inflorescence2;
- association mapping;
- quantitative trait loci;
- polar auxin transport
We characterized allelic variation at barren inflorescence2 (bif2), a maize co-ortholog of the Arabidopsis PINOID protein kinase (PID), and tested for trait associations with bif2 in both an association mapping population of 277 diverse maize inbreds and in the inter-mated B73 × Mo17 (IBM) linkage population. Results from the quantitative analyses were compared with previous reports of bif2 phenotypes in mutagenesis studies. All three approaches (association, linkage, and mutagenesis) detect a significant effect of bif2 on tassel architecture. Association mapping implicates bif2 in an unexpectedly wide range of traits including plant height, node number, leaf length, and flowering time. Linkage mapping finds a significant interaction effect for node number between bif2 and other loci, in keeping with previous reports that bif2;spi1 and Bif2;Bif1 double mutants produce fewer phytomers. The Mo17 allele is associated with a reduced tassel branch zone and shows lower expression than the B73 allele in hybrid B73–Mo17 F1 inflorescences, consistent with the complete absence of tassel branches in the bif2 knockout mutant. Overall, these data suggest that allelic variation at bif2 affects maize architecture by modulating auxin transport during vegetative and inflorescence development.