†These authors contributed equally to this work.
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Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays
Article first published online: 9 MAR 2009
DOI: 10.1111/j.1365-313X.2009.03835.x
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Additional Information
How to Cite
Zeller, G., Henz, S. R., Widmer, C. K., Sachsenberg, T., Rätsch, G., Weigel, D. and Laubinger, S. (2009), Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays. The Plant Journal, 58: 1068–1082. doi: 10.1111/j.1365-313X.2009.03835.x
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†These authors contributed equally to this work.
Publication History
- Issue published online: 10 JUN 2009
- Article first published online: 9 MAR 2009
- Received 23 September 2008; revised 16 January 2009; accepted 4 February 2009; published online 9 March 2009.
Keywords:
- tiling array;
- abiotic stress;
- Arabidopsis;
- ABA;
- At-TAX
Summary
The responses of plants to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress-induced changes in the Arabidopsis thaliana transcriptome, we have used whole-genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Among annotated genes in the reference strain Columbia we have found many stress-responsive genes, including several transcription factor genes as well as pseudogenes and transposons that have been missed in previous analyses with standard expression arrays. In addition, we report hundreds of newly identified, stress-induced transcribed regions. These often overlap with known, annotated genes. The results are accessible through the Arabidopsis thaliana Tiling Array Express (At-TAX) homepage, which provides convenient tools for displaying expression values of annotated genes, as well as visualization of unannotated transcribed regions along each chromosome.