Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set
Version of Record online: 27 MAR 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 59, Issue 1, pages 169–178, July 2009
How to Cite
Geldner, N., Dénervaud-Tendon, V., Hyman, D. L., Mayer, U., Stierhof, Y.-D. and Chory, J. (2009), Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set. The Plant Journal, 59: 169–178. doi: 10.1111/j.1365-313X.2009.03851.x
- Issue online: 25 JUN 2009
- Version of Record online: 27 MAR 2009
- Received 15 January 2009; revised 9 February 2009; accepted 19 February 2009; published online 27 March 2009.
Figure S1. Available plant transformation vector set for UPS recombination cloning.
Vectors pNIGEL07, 17, 18 and 19 have been used for this publication. Only 07, 17, 18 have been tested in plants. Vector sequences have been submitted to Genbank and Clones will be made available through the Arabidopsis Biological Resource Center (ABRC).
Figure S2. Quantitative co-localisation of YFP-tagged Wave set with the endocytic tracer FM4-64.
(a) 5–10 min of uptake, (b) 60–90 min of uptake, (c) 60 min of uptake with BFA 25 μm. YFP-tagged compartments shown in green, FM4-64 in red.
(d) Quantification of co-localisation of pictures shown in (a–c). 5–10 min FM4-64 in blue, 60–90 min in red, BFA treatment in green. This panel shows the complete set of markers analysed, a selection of which appears in Figure 3 of the publication.
Figure S3. Mapping of membrane compartments by combinatorial co-localisation.
A subset of YFP-tagged (Y) and mCherry-tagged (R) lines were chosen for crosses. YFP signals always in green, mCherry signals always in red. Low right picture block shows selected double-tagged lines after BFA treatment. Scale bar (upper left picture): 5 μm. This panel shows the complete set of markers analysed, a selection of which appears in Figure 3 of the publication.
Figure S4. Immuno-electron microscopy of Wave lines with different antibodies.
(a) Immuno-staining of Wave 33Y (RabD2b) using anti-myc antibody. Note that the staining is present in Golgi stacks, but that strongest staining is seen in the trans-Golgi network region.
(b, c) Immuno-staining of Wave 18Y (AtGot1p) using both anti-myc (b) and anti-GFP (c) antibodies. As expected both show similar localization of label to the Golgi stacks, with preferential staining of the periphery. Labeling at the trans-Golgi network can only be occasionally observed. Scale bar: 500 nm.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.