These authors contributed equally to this work.
Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants
Version of Record online: 9 JUL 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 60, Issue 1, pages 48–55, October 2009
How to Cite
Suzuki, H., Park, S.-H., Okubo, K., Kitamura, J., Ueguchi-Tanaka, M., Iuchi, S., Katoh, E., Kobayashi, M., Yamaguchi, I., Matsuoka, M., Asami, T. and Nakajima, M. (2009), Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants. The Plant Journal, 60: 48–55. doi: 10.1111/j.1365-313X.2009.03936.x
- Issue online: 24 SEP 2009
- Version of Record online: 9 JUL 2009
- Received 21 April 2009; accepted 8 May 2009; published online 9 July 2009.
Figure S1. Absolute quantification of AtGID1 (a) and DELLA (b) transcripts in ca. 300 filaments. All transcripts were absolutely quantified by qRT-PCR based on the Act2 gene. Quantification was independently repeated twice, and similar results were obtained.
Figure S2. Control experiments for competitive Y3H assay. (a), The assay for the combination of one AtGID1 as bait (BDGID1) and a DELLA protein as prey (ADDELLA) was examined on selection medium in the presence of GA4 (10 μm) and 3-AT (10-100 mM). Nothing ligated to the pMet site (2ndvec). (b) On the non-selection medium (SD without L-leucine, L-tryptophan, uracil, and L-methionine) in the absence of GA4, the assay for all BDGID1-ADDELLA-2ndGID1 combinations was examined. 3-AT (100 mm) was added. Both assays were repeated three times independently, and similar results were obtained each time.
Figure S3. Immunological detection of two GID1s in all surviving yeasts for three-hybrid assay. All BDGID1s have a molecular size of around 57 kDa (arrow head). All 2ndGID1s have a molecular size of around 40 kDa (arrow). Asterisks (*) indicate bands that reacted non-specifically with anti-GID1 antiserum.
Table S1. Sequences for primers.
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|TPJ_3936_sm_Fig S1.pdf||96K||Supporting info item|
|TPJ_3936_sm_Fig S2.pdf||3707K||Supporting info item|
|TPJ_3936_sm_Fig S3.pdf||72K||Supporting info item|
|TPJ_3936_sm_Table S1.pdf||63K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.