Lipid microdomain polarization is required for NADPH oxidase-dependent ROS signaling in Picea meyeri pollen tube tip growth

Authors

  • Peng Liu,

    1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Rui-Li Li,

    1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Liang Zhang,

    1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
    Search for more papers by this author
  • Qin-Li Wang,

    1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    Search for more papers by this author
  • Karsten Niehaus,

    1. Faculty of Biology, Department of Proteome and Metabolome Research, Bielefeld University, D-33594 Bielefeld, Germany
    Search for more papers by this author
  • František Baluška,

    1. Institute of Cellular and Molecular Botany, Rheinische Friedrich-Wilhelms-University Bonn, Department of Plant Cell Biology, Kirschallee 1, D-53115 Bonn, Germany
    Search for more papers by this author
  • Jozef Šamaj,

    1. Institute of Cellular and Molecular Botany, Rheinische Friedrich-Wilhelms-University Bonn, Department of Plant Cell Biology, Kirschallee 1, D-53115 Bonn, Germany
    2. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, SK-95007 Nitra, Slovak Republic
    3. Faculty of Science, Palacký University, 78371 Olomouc, Czech Republic
    Search for more papers by this author
  • Jin-Xing Lin

    Corresponding author
    1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
      *(fax +86 10 62836211; e-mail linjx@ibcas.ac.cn).
    Search for more papers by this author

*(fax +86 10 62836211; e-mail linjx@ibcas.ac.cn).

Summary

The polarization of sterol-enriched lipid microdomains has been linked to morphogenesis and cell movement in diverse cell types. Recent biochemical evidence has confirmed the presence of lipid microdomains in plant cells; however, direct evidence for a functional link between these microdomains and plant cell growth is still lacking. Here, we reported the involvement of lipid microdomains in NADPH oxidase (NOX)-dependent reactive oxygen species (ROS) signaling in Picea meyeri pollen tube growth. Staining with di-4-ANEPPDHQ or filipin revealed that sterol-enriched microdomains were polarized to the growing tip of the pollen tube. Sterol sequestration with filipin disrupted membrane microdomain polarization, depressed tip-based ROS formation, dissipated tip-focused cytosolic Ca2+ gradient and thereby arrested tip growth. NOX clustered at the growing tip, and corresponded with the ordered membrane domains. Immunoblot analysis and native gel assays demonstrated that NOX was partially associated with detergent-resistant membranes and, furthermore, that NOX in a sterol-dependent fashion depends on membrane microdomains for its enzymatic activity. In addition, in vivo time-lapse imaging revealed the coexistence of a steep tip-high apical ROS gradient and subapical ROS production, highlighting the reported signaling role for ROS in polar cell growth. Our results suggest that the polarization of lipid microdomains to the apical plasma membrane, and the inclusion of NOX into these domains, contribute, at least in part, to the ability to grow in a highly polarized manner to form pollen tubes.

Ancillary