Present addresses: Bayer BioScience NV, TechnologiePark 58, Gent 9052, Belgium.
Cell wall glucomannan in Arabidopsis is synthesised by CSLA glycosyltransferases, and influences the progression of embryogenesis
Version of Record online: 5 AUG 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 60, Issue 3, pages 527–538, November 2009
How to Cite
Goubet, F., Barton, C. J., Mortimer, J. C., Yu, X., Zhang, Z., Miles, G. P., Richens, J., Liepman, A. H., Seffen, K. and Dupree, P. (2009), Cell wall glucomannan in Arabidopsis is synthesised by CSLA glycosyltransferases, and influences the progression of embryogenesis. The Plant Journal, 60: 527–538. doi: 10.1111/j.1365-313X.2009.03977.x
- Issue online: 28 OCT 2009
- Version of Record online: 5 AUG 2009
- Received 27 April 2009; revised 22 June 2009; accepted 1 July 2009; published online 5 August 2009.
Figure S1. Quantitation of PACE band intensity. (a) ratio of band intensity of WT mannanase digested AIR compared to csla mutants for band ‘a’. (b) ratio of band intensity of WT mannanase digested AIR compared to csla mutants for band ‘b’. Refer to Figure 2 for location of bands. SD of two biological replicates.
Figure S2. HPAEC-PAD traces showing monosaccharide analysis of WT and csla2csla3csla9 mutant stem cell walls.
Figure S3. Four-point bending of stems: (a) miniature bending rig housed in an INSTRON tensometer; (b) schematic of the location of the tab–stem–tab specimen relative to the jig and the force, f applied to it by the load cell; (c) free-body diagram of the contacting point forces applied to the tabs and corresponding displacement, u, of their ends; (d) equivalent moment, M, applied to the ends of stem and its deformed, uniform radius of curvature, R.
Figure S4. PCR of plants showing complementation of csla7 with 35Spro:CSLA9. (1) WT DNA, gene/gene primers (2) WT DNA, gene/DS primers (3) Heterozygous csla7 gene/gene primers (4) Heterozygous csla7 gene/DS primers (5) Homozygous csla7 expressing 35Spro:CSLA9 gene/gene primers (6) Homozygous csla7 expressing 35Spro:CSLA9 gene/DS primers.
Table S1. Crosses made between different csla mutants, and the phenotype observed.
Table S2. Primers/conditions for PCR and RT-PCR, sequences are given 5′ 3′.
Appendix S1. Supplemental methods: Mechanical testing and analysis of Arabidopsis stems.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.