SEARCH

SEARCH BY CITATION

Figure S1. Quantitation of PACE band intensity. (a) ratio of band intensity of WT mannanase digested AIR compared to csla mutants for band ‘a’. (b) ratio of band intensity of WT mannanase digested AIR compared to csla mutants for band ‘b’. Refer to Figure 2 for location of bands. SD of two biological replicates.

Figure S2. HPAEC-PAD traces showing monosaccharide analysis of WT and csla2csla3csla9 mutant stem cell walls.

Figure S3. Four-point bending of stems: (a) miniature bending rig housed in an INSTRON tensometer; (b) schematic of the location of the tab–stem–tab specimen relative to the jig and the force, f applied to it by the load cell; (c) free-body diagram of the contacting point forces applied to the tabs and corresponding displacement, u, of their ends; (d) equivalent moment, M, applied to the ends of stem and its deformed, uniform radius of curvature, R.

Figure S4. PCR of plants showing complementation of csla7 with 35Spro:CSLA9. (1) WT DNA, gene/gene primers (2) WT DNA, gene/DS primers (3) Heterozygous csla7 gene/gene primers (4) Heterozygous csla7 gene/DS primers (5) Homozygous csla7 expressing 35Spro:CSLA9 gene/gene primers (6) Homozygous csla7 expressing 35Spro:CSLA9 gene/DS primers.

Table S1. Crosses made between different csla mutants, and the phenotype observed.

Table S2. Primers/conditions for PCR and RT-PCR, sequences are given 5′ [RIGHTWARDS ARROW] 3′.

Appendix S1. Supplemental methods: Mechanical testing and analysis of Arabidopsis stems.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
TPJ_3977_sm_Fig_S1-S4.doc618KSupporting info item
TPJ_3977_sm_Methods.doc52KSupporting info item
TPJ_3977_sm_Table_S1-S2.doc53KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.