Genotype and time of day shape the Populus drought response
Article first published online: 1 SEP 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 60, Issue 4, pages 703–715, November 2009
How to Cite
Wilkins, O., Waldron, L., Nahal, H., Provart, N. J. and Campbell, M. M. (2009), Genotype and time of day shape the Populus drought response. The Plant Journal, 60: 703–715. doi: 10.1111/j.1365-313X.2009.03993.x
- Issue published online: 12 NOV 2009
- Article first published online: 1 SEP 2009
- Received 18 March 2009; revised 17 June 09; accepted 28 July 2009; published online 1 September 2009.
- hybrid poplar;
As exposure to episodic drought can impinge significantly on forest health and the establishment of productive tree plantations, there is great interest in understanding the mechanisms of drought response in trees. The ecologically dominant and economically important genus Populus, with its sequenced genome, provides an ideal opportunity to examine transcriptome level changes in trees in response to a drought stimulus. The transcriptome level drought response of two commercially important Populus clones (P. deltoides × P. nigra, DN34, and P. nigra × P. maximowiczii, NM6) was characterized over a diurnal period using a 4 × 2 × 2 complete randomized factorial anova experimental design (four time points, two genotypes and two treatment conditions), using Affymetrix Poplar GeneChip microarrays. Notably, the specific genes that exhibited changes in transcript abundance in response to drought differed between the genotypes and/or the time of day that they exhibited their greatest differences. This study emphasizes the fact that it is not possible to draw simple, generalized conclusions about the drought response of the genus Populus on the basis of one species, nor on the basis of results collected at a single time point. The data derived from our studies provide insights into the variety of genetic mechanisms underpinning the Populus drought response, and provide candidates for future experiments aimed at understanding this response across this economically and ecologically important genus.