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Figure S1. Virus Induced Gene Silencing (VIGS) of TAG1 resulted in yellow-orange sectors upon fruit maturation. (a) Ripe fruit of a plant infected with a vector containing the full TAG1 (b) Ripe fruit of plant infected with vector carrying a full TAG1 sequence fragment. (c) Ripe fruit of plant infected with the positive control vector, carrying a specific fragment of the PHYTOENE DESATURASE (PDS).

Figure S2. Overexpression of TAGL1 as a chimeric repressor (TAGL1-SRDX) resulted in a reduction in CO2 emission from fruit of transgenic plants. CO2 emission from TAGL1-SRDX and wild type (wt) fruit was measured as described in Experimental Procedures. Asterisk indicates p-value <0.05 (Stuudent’s t-test; = 3).

Figure S3.TAGL1 overexpression in fruit induces inflation and ripening of sepals. (a) Ripe wild type fruit. (b) Ripe rin fruit. (c) TAGL1 overexpression (TAGL1oe) on wt background results in inflated sepals with reddish regions of lycopene accumulation (indicated by an arrow; see Figure 6a) (d) rinTAGL1oe (rin mutant background) results in inflated sepals with yellow regions of naringenin chalcone accumulation (indicated by an arrow; see Figure 6d). (e) and (f) Ripe fruits of two F1 segregating lines derived from the cross between rinTAGL1oe and wild type.

Figure S4. Overexpression of TAGL1 in the wt background has no effect on the levels of most isoprenoids in sepals. The levels of isoprenoids in sepals of TAGL1oe compared to wt stay unaltered, except for lycopene (see Figure 6a). This might be related to the mild phenotype of the analysed sepals. Isoprenoids were extracted and measured as described in Experimental Procedures from pools of MicroTom Ripe fruit (n = 2–7). Compounds were identified using UV/visible spectra of standards and known retention time.

Figure S5.TAGL1 relative transcript levels in wt, rin mutant and transgenic lines in the rin background. TAGL1 transcript levels are significantly altered in fruit (rin background; Br) of transgenic lines demonstrating overexpression and co-suppression phenotypes (see Figure 6.). Similar TAGL1 expression levels were observed in Br fruits of wt and rin in the cv. MicroTom background.

Figure S6. Levels of naringenin chalcone (NarCh) in the peel of wt and rin ripe fruit. The levels of naringenin chalcone are significantly higher in the peel of wt fruit compared to the peel of rin fruit.

Figure S7. Sepals of TAGL1 overexpressing lines remain fused all along flowering. (a) Wild type flowers. (b) The rin mutant flowers. (c) The TAGL1oe flowers. (d) The rinTAGL1oe flowers. Fused sepals in transgenic plants are indicated with an arrow.

Table S1. List of genes showing altered expression in TAGL1-SRDX breaker fruit.

Table S2. Oligonucleotides that were used in this study.

Table S3. Full names and identifiers of protein sequences used in the phylogenetic analysis.

Table S4. Isoprenoids detected by HPLC analysis.

Appendix S1. Supplementary experimental procedures.

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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.