These authors contributed equally to this work.
The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing
Article first published online: 19 NOV 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
The Plant Journal
Volume 61, Issue 3, pages 456–466, February 2010
How to Cite
Tang, J., Kobayashi, K., Suzuki, M., Matsumoto, S. and Muranaka, T. (2010), The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing. The Plant Journal, 61: 456–466. doi: 10.1111/j.1365-313X.2009.04082.x
Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://www3.interscience.wiley.com/authorresources/onlineopen.html
- Issue published online: 21 JAN 2010
- Article first published online: 19 NOV 2009
- Received 6 August 2009; revised 21 October 2009; accepted 30 October 2009; published online 21 December 2009.
Figure S1. Defects in RNA editing of three mitochondrial genes observed in loi1-2. Direct sequencing of RT-PCR products of nad4, ccb203, and cox3 from WT, loi1-1, and loi1-2. Sequence direction is shown in the figure. Nucleotides edited in WT but not in loi1-1 and loi1-2 are shown by arrows.
Figure S2. Phenotypes of WT treated with lovastatin and clomazone combined with SHAM.
Figure S3. Quantitative data on the effect of inhibitors of the respiratory chain on lovastatin sensitivity.
Figure S4. Total chlorophyll content of seedlings grown with inhibitors of the respiratory chain combined with clomazone. Chlorophylls were extracted from 1-week-old seedlings and quantified by measuring absorbance at A646 and A663 (Harborne, 1998).
Figure S5. Expression analysis of AOX1a. Transcription of AOX1a was examined with RT-PCR using cDNA from 1-week-old WT and loi1-1 seedlings grown with or without lovastatin. EF-1a was amplified as a control. RT-PCR was performed using the following primers: AOX1a-fwd, 5’-CTGTTAGTGGCGGCTGGACCACGTT-3’; AOX1a-rev, 5’-GTTCACGACCTTGGTAGTGAATATCA-3’; EF-1α-fwd, 5’-ACACATTCTTCCTCCGCATCATCCT-3’; and EF-1α-rev, 5’-TGGCATCCATCTTGTTACAACAGCAG-3’.
Figure S6. Sequence alignment of cDNA, starting from –45 to +10, of three unedited C residues of loi1. Red T was generated by RNA editing. Conserved nucleotide bases of all three cDNAs are shown in pink. Conserved nucleotide bases of two RNAs are shown in green (nad4 and ccb203), aqua (ccb203 and cox3), and yellow (nad4 and cox3).
Table S1. Comparison of RNA editing between WT and loi1-1. Primer information using direct sequencing. The loci of the RNA editing sites are also shown according to TAIR (http://www.arabidopsis.org). The fully or partially edited sites in our study are shown in black, and non-edited sites are shown in grey.
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|TPJ_4082_sm_Supporting_Information.doc||29K||Supporting info item|
|TPJ_4082_sm_supplemental_table1.pdf||37K||Supporting info item|
|TPJ_4082_sm_Supplement_data.pdf||5368K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.