H2O2 in plant peroxisomes: an in vivo analysis uncovers a Ca2+-dependent scavenging system
Article first published online: 2 MAR 2010
© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd
The Plant Journal
Volume 62, Issue 5, pages 760–772, June 2010
How to Cite
Costa, A., Drago, I., Behera, S., Zottini, M., Pizzo, P., Schroeder, J. I., Pozzan, T. and Schiavo, F. L. (2010), H2O2 in plant peroxisomes: an in vivo analysis uncovers a Ca2+-dependent scavenging system. The Plant Journal, 62: 760–772. doi: 10.1111/j.1365-313X.2010.04190.x
- Issue published online: 25 MAY 2010
- Article first published online: 2 MAR 2010
- Received 14 January 2010; revised 9 February 2010; accepted 16 February 2010; published online 25 March 2010.
- plant peroxisomes;
- hydrogen peroxide
Oxidative stress is a major challenge for all cells living in an oxygen-based world. Among reactive oxygen species, H2O2, is a well known toxic molecule and, nowadays, considered a specific component of several signalling pathways. In order to gain insight into the roles played by H2O2 in plant cells, it is necessary to have a reliable, specific and non-invasive methodology for its in vivo detection. Hence, the genetically encoded H2O2 sensor HyPer was expressed in plant cells in different subcellular compartments such as cytoplasm and peroxisomes. Moreover, with the use of the new green fluorescent protein (GFP)-based Cameleon Ca2+ indicator, D3cpv–KVK–SKL, targeted to peroxisomes, we demonstrated that the induction of cytoplasmic Ca2+ increase is followed by Ca2+ rise in the peroxisomal lumen. The analyses of HyPer fluorescence ratios were performed in leaf peroxisomes of tobacco and pre- and post-bolting Arabidopsis plants. These analyses allowed us to demonstrate that an intraperoxisomal Ca2+ rise in vivo stimulates catalase activity, increasing peroxisomal H2O2 scavenging efficiency.