The SERK1 receptor-like kinase regulates organ separation in Arabidopsis flowers
Article first published online: 2 MAR 2010
© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd
The Plant Journal
Volume 62, Issue 5, pages 817–828, June 2010
How to Cite
Lewis, M. W., Leslie, M. E., Fulcher, E. H., Darnielle, L., Healy, P. N., Youn, J.-Y. and Liljegren, S. J. (2010), The SERK1 receptor-like kinase regulates organ separation in Arabidopsis flowers. The Plant Journal, 62: 817–828. doi: 10.1111/j.1365-313X.2010.04194.x
- Issue published online: 25 MAY 2010
- Article first published online: 2 MAR 2010
- Received 6 October 2009; revised 18 January 2010; accepted 17 February 2010; published online 16 April 2010.
- receptor-like kinase;
- membrane trafficking;
- flower development
Through a sensitized screen for novel components of pathways regulating organ separation in Arabidopsis flowers, we have found that the leucine-rich repeat receptor-like kinase SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) acts as a negative regulator of abscission. Mutations in SERK1 dominantly rescue abscission in flowers without functional NEVERSHED (NEV), an ADP-ribosylation factor GTPase-activating protein required for floral organ shedding. We previously reported that the organization of the Golgi apparatus and location of the trans-Golgi network (TGN) are altered in nev mutant flowers. Disruption of SERK1 restores Golgi structure and the close association of the TGN in nev flowers, suggesting that defects in these organelles may be responsible for the block in abscission. We have also found that the abscission zones of nev serk1 flowers are enlarged compared to wild-type. A similar phenotype was previously observed in plants constitutively expressing a putative ligand required for organ separation, INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), suggesting that signalling through IDA and its proposed receptors, HAESA and HAESA-LIKE2, may be deregulated in nev serk1 abscission zone cells. Our studies indicate that in addition to its previously characterized roles in stamen development and brassinosteroid perception, SERK1 plays a unique role in modulating the loss of cell adhesion that occurs during organ abscission.