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Figure S1. CW staining of 11 mutant lines selected from a list of 46 genes up-regulated in leaf tissue subjected to abiotic stresses known to cause elevated levels of wall ingrowth deposition in PP TCs of veins. All mutant lines, with the exception of GIGANTEA, showed levels of CW stained PP in veins equivalent to that seen in WT (Col-0). The AGI and ABRC stock numbers for each gene and mutant line, respectively, are as follows: MYB TF1, MYB family transcription factor 1 (At1g01520; SALK_076666C); MYB TF2, MYB family transcription factor 2 (At1g25550; SALK_035429C); ELIP2, Early Light Inducible Protein 2 (At4g14690; SALK_105392C); ATSUC1, Arabidopsis Sucrose Transporter 1 (At1g71880; CS26561); CDK19, Calcium-Dependant Kinase 19 (At1g61950; SALK_057587C); NRT2.1, High-Affinity Nitrate Transporter 2.1 (At1g08090; SALK_035429C); Phospholipase (At3g15650; SALK_093573C); REF1, Rubber Elongation Factor 1 (At1g67360; SALK_148081C); CML37, Calmodulin-Like Protein 37 (At5g42380; SALK_011488C); Chalcone synthase (At5g13930; CS8605); GIGANTEA (At1g22770; CS3124). Scale bar = 50 μm.

Figure S2. Mature rosette leaves from WT (top) and gi-2 (bottom) showing no substantial differences in leaf morphology or venation patterning. Leaves were stained with 0.01% (w/v) saffranin O according to Haritatos et al. (2000).

Table S1. Microarray data sets used to identify commonly up-regulated and down-regulated genes under different abiotic stress conditions that most closely resemble those known to induce wall ingrowth development in PP-TCs of leaf minor veins in Arabidopsis. Information listed here includes treatment conditions, data set replicates, NASC ID codes for each data set, type of cDNA microarray chip used to create the data set and the primary contributor.

Table S2. List of genes commonly up-regulated two-fold or more in Arabidopsis leaf tissue within 24 h exposure to high light or cold. This list was generated by analysing the microarray data sets listed in Table S1. The average fold increase in expression of each gene across the six data sets is indicated.

Table S3. List of genes commonly down-regulated two-fold or more in Arabidopsis leaf tissue within 24 h exposure to high light or cold. This list was generated by analysing the microarray data sets listed in Table S1. The average fold decrease in expression of each gene across the six data sets is indicated.

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