These authors contributed equally to this work.
The PAM1 gene of petunia, required for intracellular accommodation and morphogenesis of arbuscular mycorrhizal fungi, encodes a homologue of VAPYRIN
Article first published online: 28 SEP 2010
© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd
The Plant Journal
Volume 64, Issue 3, pages 470–481, November 2010
How to Cite
Feddermann, N., Duvvuru Muni, R. R., Zeier, T., Stuurman, J., Ercolin, F., Schorderet, M. and Reinhardt, D. (2010), The PAM1 gene of petunia, required for intracellular accommodation and morphogenesis of arbuscular mycorrhizal fungi, encodes a homologue of VAPYRIN. The Plant Journal, 64: 470–481. doi: 10.1111/j.1365-313X.2010.04341.x
- Issue published online: 26 OCT 2010
- Article first published online: 28 SEP 2010
- Accepted manuscript online: 23 AUG 2010 09:50AM EST
- Received 17 June 2010; revised 10 August 2010; accepted 17 August 2010; published online 28 September 2010.
- Glomus intraradices;
- Petunia hybrida;
- arbuscular mycorrhiza;
- intracellular accommodation
Most terrestrial plants engage into arbuscular mycorrhizal (AM) symbiosis with fungi of the phylum Glomeromycota. The initial recognition of the fungal symbiont results in the activation of a symbiosis signalling pathway that is shared with the root nodule symbiosis (common SYM pathway). The subsequent intracellular accommodation of the fungus, and the elaboration of its characteristic feeding structures, the arbuscules, depends on a genetic programme in the plant that has recently been shown to involve the VAPYRIN gene in Medicaco truncatula. We have previously identified a mutant in Petunia hybrida, penetration and arbuscule morphogenesis 1 (pam1), that is defective in the intracellular stages of AM development. Here, we report on the cloning of PAM1, which encodes a VAPYRIN homologue. PAM1 protein localizes to the cytosol and the nucleus, with a prominent affinity to mobile spherical structures that are associated with the tonoplast, and are therefore referred to as tonospheres. In mycorrhizal roots, tonospheres were observed in the vicinity of intracellular hyphae, where they may play an essential role in the accommodation and morphogenesis of the fungal endosymbiont.