Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules
Article first published online: 8 OCT 2010
© 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd
The Plant Journal
Volume 64, Issue 4, pages 657–667, November 2010
How to Cite
Yoneda, A., Ito, T., Higaki, T., Kutsuna, N., Saito, T., Ishimizu, T., Osada, H., Hasezawa, S., Matsui, M. and Demura, T. (2010), Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules. The Plant Journal, 64: 657–667. doi: 10.1111/j.1365-313X.2010.04356.x
- Issue published online: 10 NOV 2010
- Article first published online: 8 OCT 2010
- Accepted manuscript online: 15 SEP 2010 02:40PM EST
- Received 21 April 2010; revised 22 August 2010; accepted 31 August 2010; published online 8 October 2010.
- cellulose microfibril;
- cortical microtubule;
- chemical genetics
Cellulose and pectin are major components of primary cell walls in plants, and it is believed that their mechanical properties are important for cell morphogenesis. It has been hypothesized that cortical microtubules guide the movement of cellulose microfibril synthase in a direction parallel with the microtubules, but the mechanism by which this alignment occurs remains unclear. We have previously identified cobtorin as an inhibitor that perturbs the parallel relationship between cortical microtubules and nascent cellulose microfibrils. In this study, we searched for the protein target of cobtorin, and we found that overexpression of pectin methylesterase and polygalacturonase suppressed the cobtorin-induced cell-swelling phenotype. Furthermore, treatment with polygalacturonase restored the deposition of cellulose microfibrils in the direction parallel with cortical microtubules, and cobtorin perturbed the distribution of methylated pectin. These results suggest that control over the properties of pectin is important for the deposition of cellulose microfibrils and/or the maintenance of their orientation parallel with the cortical microtubules.