Present address: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
Evolutionary trends in the floral transcriptome: insights from one of the basalmost angiosperms, the water lily Nuphar advena (Nymphaeaceae)
Version of Record online: 8 OCT 2010
© 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd
The Plant Journal
Volume 64, Issue 4, pages 687–698, November 2010
How to Cite
Yoo, M.-J., Chanderbali, A. S., Altman, N. S., Soltis, P. S. and Soltis, D. E. (2010), Evolutionary trends in the floral transcriptome: insights from one of the basalmost angiosperms, the water lily Nuphar advena (Nymphaeaceae). The Plant Journal, 64: 687–698. doi: 10.1111/j.1365-313X.2010.04357.x
- Issue online: 10 NOV 2010
- Version of Record online: 8 OCT 2010
- Accepted manuscript online: 16 SEP 2010 10:09AM EST
- Received 28 May 2010; revised 26 August 2010; accepted 7 September 2010; published online 8 October 2010.
Figure S1. Number of genes up-regulated in Nuphar floral tissues at identified five regulatory modules.
Figure S2. Comparison of microarray data with RQ-RT-PCR-data. (a) Microarray data of AP1, AP3-1, AP3-2, and PI. For AP3, two different probes AP3-1 and AP3-2 were designed from two AP3 genes AP3-1 and AP3-2, respectively. (b) RQ-RT-PCR data of AP1 (Yoo et al., 2010), AP3-1, AP3-2, and PI (Kim et al., 2005). (c) Microarray data of AG, AGL2-a, AGL2-b, and AGL6. For AGL2, two different probes were designed from one sequence. (d) RQ-RT-PCR data of AG (Yoo et al., 2010), AGL2 (Zahn et al., 2005), AGL6 (Yoo et al., 2010). For all RQ-RT-PCR data, the expression level was relative to 18S rRNA internal control, so the maximum expression level is 1. OTE, OT = outer ‘sepaloid’ tepals, ITE, IT = inner ‘petaloid’ tepals, SD = staminodes, ST, SN = stamens, CA = carpels, LF, LE = leaves.
Figure S3. Double-loop design for Nuphar microarray experiments. Each line indicates a single array, and different color means different biological replicates. The tissue at the head of the arrow is labeled red, and the tissue at the tail is labeled green.
Table S1. Genes up-regulated in reproductive organs of Nuphar. TFs, transcription factors; Mbd, medium-aged floral buds at the pre-meiotic stage; Ybd, young floral buds at the pre-microsporangia initiation stage.
Table S2. Genes down-regulated in reproductive organs of Nuphar. TFs, transcription factors; Mbd, medium-aged floral buds at the pre-meiotic stage; Ybd, young floral buds at the pre-microsporangia initiation stage.
Table S3. List of genes showing similar expression patterns between Nuphar and Arabidopsis. Log2 transformed expression level relative to leaf tissue were shown for Nuphar and Arabidopsis.
Table S4. Number of genes up-regulated in any floral tissue of Nuphar, Persea, and Arabidopsis relative to leaves. Sepals: sepaloid outer tepals, outer tepals, and sepals. Petals: petaloid inner tepals, inner tepals, and petals.
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