The DUF579 domain containing proteins IRX15 and IRX15-L affect xylan synthesis in Arabidopsis
Article first published online: 2 FEB 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 66, Issue 3, pages 387–400, May 2011
How to Cite
Jensen, J. K., Kim, H., Cocuron, J.-C., Orler, R., Ralph, J. and Wilkerson, C. G. (2011), The DUF579 domain containing proteins IRX15 and IRX15-L affect xylan synthesis in Arabidopsis. The Plant Journal, 66: 387–400. doi: 10.1111/j.1365-313X.2010.04475.x
- Issue published online: 26 APR 2011
- Article first published online: 2 FEB 2011
- Accepted manuscript online: 28 DEC 2010 01:23AM EST
- Received 24 August 2010; revised 20 November 2010; accepted 21 December 2010.
- secondary cell wall;
- degree of polymerization
Xylan is the principal hemicellulose in the secondary cell walls of eudicots and in the primary and secondary cell walls of grasses and cereals. The biosynthesis of this important cell wall component has yet to be fully determined although a number of proteins have been shown to be required for xylan synthesis. To discover new genes involved in xylan biosynthesis we explored the psyllium (Plantago ovata Forsk) seed mucilaginous layer through EST profiling. This tissue synthesizes large amounts of a complex heteroxylan over a short period of time. By comparing abundant transcripts in this tissue with abundant transcripts specifically present during secondary cell wall formation in Arabidopsis thaliana, where glucuronoxylan biosynthesis is pronounced, we identified two Arabidopsis genes likely involved in xylan biosynthesis. These genes encode proteins containing a Domain of Unknown Function (DUF) 579 and were designated IRREGULAR XYLEM (IRX) 15 and IRX15-LIKE (IRX15-L). We obtained Arabidopsis T-DNA knockout lines for the two genes and analyzed their lower stems for changes in neutral monosaccharide composition. No changes were observed in each of these mutants, although the irx15 irx15-L double mutant displayed a moderate reduction in stem xylose. Further characterization of the irx15 irx15-L mutant revealed irregular secondary cell wall margins in fiber cells and a lower xylan degree of polymerization. Through these studies we conclude that IRX15 and IRX15-L function in a redundant manner and are involved in xylan biosynthesis.