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Figure S1. Alignment of the predicted amino acid sequence of the 10 Arabidopsis DUF579-containing genes. Alignment and output are from ClustalX. Large boxed area show the extent of the DUF579 domain. Smaller boxed areas show individual predicted transmembrane spanning domains.

Figure S2. The relationship between different DUF579 containing genes and their tissue specific expression in roots. The Cladogram was generated by TreeView X (R. Page, University of Glasgow) using the phylip tree output of ClustalW2. Shown for each DUF579 member is a graphical representation of cell type expression data from Brady et al. (2007) Science, 318, 801–806. Root tip expression is viewed towards the front of the graph with late developing tissue towards the rear. A spatial expression profile is shown for the secondary wall cellulose synthase subunit-encoding CESA7 showing expression to be restricted to a peak at the rear center of the representation corresponding to late xylem development. Five of the DUF679 members, distributed between two distinct clades (labelled I and II), are found to have closely matched spatial expression profiles to CESA7.

Figure S3. Characterisation of mutant lines. (A) T-DNA insertion lines and gene models for DUF579 containing gene families. Approximate position of the T-DNA insertions are indicated. Introns and 300 bp of both the 3′ and 5′UTR are indicated by grey lines. (B). Measurement of IRX15 and IRX15L mRNA transcripts in mutant and wild type plants. Expression of IRX15 (closed bars) and IRX15L (open bars) are represented as a percentage of actin expression. Standard error bars are shown (n = 3).

Figure S4. Whole plant phenotype of wild type and irx15 irx15l lines.

Figure S5. Example PACE gels used to quantify xylan levels. The results of the analysis are shown graphically in Figure 4A.

Table S1. DUF579-containing genes and insertion lines used in this study.

Video Clip S1. Timelapse of YFP: IRX15L in a developing xylem vessel. Four small compartments were tracked using the ImageJ Manual Tracking plugin (F. Cordelieres, Institut Curie) and their pathways are drawn in the lower panel.

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