These authors contributed equally to this work.
Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root
Article first published online: 1 MAR 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 66, Issue 3, pages 516–527, May 2011
How to Cite
Zhou, Z.-Y., Zhang, C.-G., Wu, L., Zhang, C.-G., Chai, J., Wang, M., Jha, A., Jia, P.-F., Cui, S.-J., Yang, M., Chen, R. and Guo, G.-Q. (2011), Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root. The Plant Journal, 66: 516–527. doi: 10.1111/j.1365-313X.2011.04509.x
- Issue published online: 26 APR 2011
- Article first published online: 1 MAR 2011
- Accepted manuscript online: 22 JAN 2011 07:43AM EST
- Received 6 May 2010; revised 12 January 2011; accepted 19 January 2011.
- auxin biosynthesis and transport;
- root curling;
Cytokinin (CK) influences many aspects of plant growth and development, and its function often involves intricate interactions with other phytohormones such as auxin and ethylene. However, the molecular mechanisms underlying the role of CK and its interactions with other growth regulators are still poorly understood. Here we describe the isolation and characterization of the Arabidopsis CK-induced root curling 1 (ckrc1) mutant. CKRC1 encodes a previously identified tryptophan aminotransferase (TAA1) involved in the indole-3-pyruvic acid (IPA) pathway of indole-3-acetic acid (IAA) biosynthesis. The ckrc1 mutant exhibits a defective root gravitropic response (GR) and an increased resistance to CK in primary root growth. These defects can be rescued by exogenous auxin or IPA. Furthermore, we show that CK up-regulates CKRC1/TAA1 expression but inhibits polar auxin transport in roots in an AHK3/ARR1/12-dependent and ethylene-independent manner. Our results suggest that CK regulates root growth and development not only by down-regulating polar auxin transport, but also by stimulating local auxin biosynthesis.