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Figure S1. High-performance LC analysis of the products from the rice CYP734A2 assay with 22,23-hydroxylated brassinosteroid s as substrates.

Figure S2. Total ion chromatogram of products from the CYP734A2 assay with 22-hydroxylated brassinosteroid s as substrates.

Figure S3. Gas chromatography retention time and mass spectrum of products from the CYP734A2 assay with castasterone as substrate.

Figure S4. Multiple alignment of amino acid sequences of the CYP734A subfamily from various plants.

Table S1. Amino acid sequence identities among CYP734A enzymes.

Table S2. Primer sequences used in CYP734A cDNA cloning.

Table S3. Primer sequences used in expression analysis.

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