Present address: 122 Plant Biology Lab, Michigan State University, East Lansing, MI 48824-1319, USA.
Cryptochrome 1 and phytochrome B control shade-avoidance responses in Arabidopsis via partially independent hormonal cascades
Version of Record online: 25 MAY 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 67, Issue 2, pages 195–207, July 2011
How to Cite
Keller, M. M., Jaillais, Y., Pedmale, U. V., Moreno, J. E., Chory, J. and Ballaré, C. L. (2011), Cryptochrome 1 and phytochrome B control shade-avoidance responses in Arabidopsis via partially independent hormonal cascades. The Plant Journal, 67: 195–207. doi: 10.1111/j.1365-313X.2011.04598.x
- Issue online: 13 JUL 2011
- Version of Record online: 25 MAY 2011
- Accepted manuscript online: 2 APR 2011 10:16AM EST
- Received 30 January 2011; revised 28 March 2011; accepted 31 March 2011; published online 25 May 2011.
Figure S1. SAS responses to B light attenuation are conserved in quintuple yucca mutants. Plants were grown under white light (WL) or WL filtered through a yellow filter (-BLUE). Morphological measurements and photographs were taken after 7 days of treatment. Thin bars indicate ±1 SE (n = 10–12 individual plants). Asterisks indicate significant effects of the light treatments at the indicated P value. (a–c) Comparison of morphological responses between Col-0 and quintuple yucca mutants. (d) Representative photographs of the plants after exposure to the indicated light treatments.
Figure S2. Effect of NPA on leaf angle, petiole length and L:P ratio of WT Columbia-0 plants in response to low blue treatment. NPA was sprayed onto intact 14-d rosette plants immediately before the beginning of the light treatments. After the NPA treatment, the plants were grown under white light (WL, white bars) or WL filtered through a yellow filter (-BLUE, black bars). Morphological measurements and photographs were taken after 4 days of treatment. Thin bars indicate ±1SE (n = 10–12 individual plants). Asterisks indicate significant effects of the light treatments at the indicated P value. Different letters indicate significant differences between means in cases in which the light x genotype interaction term (L × G) was significant. (a–c) NPA effect at high (50 μM) doses. This NPA treatment caused considerably growth inhibition and leaf curling, bud did not prevent the hyponastic response. (d–f) NPA effect at low doses (≤5 μM). Notice that these low NPA doses had per se a positive effect promoting a hyponastic response.
Figure S3. GA treatment triggered rapid mCITRINE-RGA turnover in the pUBQ10::mCITRINE-RGA line.
Figure S4. Representative photographs of the phenotype of the det2-1 mutant under the attenuated blue light conditions used in this study. Photographs were taken after 7 days of treatment.
Figure S5. Irradiances and spectral photon distributions under the light treatments used in the canopy and filtration experiments, respectively. (a) Mean and Standard Error values for the PAR and B irradiances (μmoles.m−2.s−1) and the R:FR ratio characteristic of light environments in the glasshouse canopy experiments shown in Figure 2. (b) Spectral photon distributions under the light treatments used in the growth chamber filtration experiments.
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