The APC/C subunit 10 plays an essential role in cell proliferation during leaf development

Authors

  • Nubia B. Eloy,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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  • Marcelo de Freitas Lima,

    1. Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, CCS, Cidade Universitária – Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brasil
    2. Departamento de Química, Universidade Federal Rural do Rio de Janeiro, CEP 23890-000, Seropédica, RJ, Brasil
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  • Daniël Van Damme,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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  • Hannes Vanhaeren,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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  • Nathalie Gonzalez,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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  • Liesbeth De Milde,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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  • Adriana S. Hemerly,

    1. Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, CCS, Cidade Universitária – Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brasil
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  • Gerrit T. S. Beemster,

    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
    3. Department of Biology, University of Antwerp, 2020 Antwerpen, Belgium
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  • Dirk Inzé,

    Corresponding author
    1. Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium
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    • These authors contributed equally to this work.

  • Paulo C. G. Ferreira

    1. Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica, CCS, Cidade Universitária – Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brasil
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    • These authors contributed equally to this work.


(fax +32 9 3313809; e-mail dirk.inze@psb.vib-ugent.be).

Summary

The largest E3 ubiquitin-ligase complex, known as anaphase-promoting complex/cyclosome (APC/C), regulates the proteolysis of cell cycle regulators such as CYCLIN B and SECURIN that are essential for sister-chromatid separation and exit from mitosis. Despite its importance, the role of APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, the Arabidopsis thaliana APC/C subunit APC10 was characterized and shown to functionally complement an apc10 yeast mutant. The APC10 protein was located in specific nuclear bodies, most probably resulting from its association with the proteasome complex. An apc10 Arabidopsis knockout mutant strongly impaired female gametogenesis. Surprisingly, constitutive overexpression of APC10 enhanced leaf size. Through kinematic analysis, the increased leaf size was found to be due to enhanced rates of cell division during the early stages of leaf development and, at the molecular level, by increased APC/C activity as measured by an amplification of the proteolysis rate of the mitotic cyclin, CYCB1;1.

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