S.H.-S. and H.E.M. contributed equally to this work.
Cellulose synthesis via the FEI2 RLK/SOS5 pathway and CELLULOSE SYNTHASE 5 is required for the structure of seed coat mucilage in Arabidopsis
Article first published online: 10 OCT 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 68, Issue 6, pages 941–953, December 2011
How to Cite
Harpaz-Saad, S., McFarlane, H. E., Xu, S., Divi, U. K., Forward, B., Western, T. L. and Kieber, J. J. (2011), Cellulose synthesis via the FEI2 RLK/SOS5 pathway and CELLULOSE SYNTHASE 5 is required for the structure of seed coat mucilage in Arabidopsis. The Plant Journal, 68: 941–953. doi: 10.1111/j.1365-313X.2011.04760.x
- Issue published online: 13 DEC 2011
- Article first published online: 10 OCT 2011
- Accepted manuscript online: 24 AUG 2011 01:05PM EST
- Received 26 May 2011; revised 2 August 2011; accepted 22 August 2011; published online 10 October 2011.
Figure S1. Scheme of mucilage secreting cell (MSC) indicating the terminology of seed and mucilage.
Figure S2.fei1 fei2 mutant seeds display seed coat mucilage defect upon water hydration.
Figure S3. Complementation of fei fei2 seed phenotype by WT and kinase-inactive FEI2.
Figure S4. Germination of fei2 vs. wild-type seeds on increasing PEG concentrations.
Figure S5. Comparison of FEI2 vs. FEI1 expression during seed development.
Figure S6. Autofluorescence of Arabidopsis seeds.
Figure S7.fei2 mutant seeds display two types of seed coat mucilage phenotypes.
Figure S8.sos5 mutant seeds show no alterations in secondary cell wall morphology of MSCs as observed by toluidine blue stained sections, when compared to wild-type.
Figure S9.fei2 and sos5 mutant seeds do not display alterations in MSCs secondary cell wall as observed by SEM.
Figure S10. Seed coat mucilage defect of three independent cesA5 mutants.
Figure S11. The seed coat mucilage defect of mum3, mum5 and sbt1.7.
Table S1. Quantification of soluble mucilage monosaccharides from fei1-1 fei2-1, sos5-2 and cesa5 versus wild-type seeds.
Table S2. Monosaccharide composition of soluble mucilage from wild-type, fei1 fei2, sos5-2 and cesA5 seeds presented as mol percent.
Data S1. Additional information regarding the chemical analysis procedures.
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|TPJ_4760_sm_FigS1.TIF||42K||Supporting info item|
|TPJ_4760_sm_FigS10.TIF||394K||Supporting info item|
|TPJ_4760_sm_FigS11.TIF||713K||Supporting info item|
|TPJ_4760_sm_FigS2.TIF||543K||Supporting info item|
|TPJ_4760_sm_FigS3.TIF||590K||Supporting info item|
|TPJ_4760_sm_FigS4.TIF||85K||Supporting info item|
|TPJ_4760_sm_FigS5.TIF||330K||Supporting info item|
|TPJ_4760_sm_FigS6.TIF||346K||Supporting info item|
|TPJ_4760_sm_FigS7.TIF||452K||Supporting info item|
|TPJ_4760_sm_FigS8.TIF||840K||Supporting info item|
|TPJ_4760_sm_FigS9.TIF||514K||Supporting info item|
|TPJ_4760_sm_SuppFigure1.pdf||42K||Supporting info item|
|TPJ_4760_sm_MethodsS1.doc||32K||Supporting info item|
|TPJ_4760_sm_SuppLegends.doc||36K||Supporting info item|
|TPJ_4760_sm_Legend.doc||48K||Supporting info item|
|TPJ_4760_sm_TablesS1-S2.doc||43K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.