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Figure S1. Detailed representation of venation segmentation steps.

(a) Segmentation of the venation pattern after grayscale dilation and thresholding of the leaf picture with removed background.

(b) Removal of small structures disconnected from the vascular network (see arrowheads in a).

(c) Thinning of the resulting binary image and removal of small spurs (see arrowheads in b). Panels are zoom-ins of Figure 2g–i.

Figure S2. Branching point and endpoint density during leaf development.

Error bars represent SE (n ≥ 10).

Table S1. Vascular analysis of wild-type plants and six transgenic lines, with contrasting cellular and leaf size phenotypes at 24 DAS.

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