These authors contributed equally to this work.
Quantitative analysis of venation patterns of Arabidopsis leaves by supervised image analysis
Version of Record online: 8 NOV 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 69, Issue 3, pages 553–563, February 2012
How to Cite
Dhondt, S., Van Haerenborgh, D., Van Cauwenbergh, C., Merks, R. M. H., Philips, W., Beemster, G. T. S. and Inzé, D. (2012), Quantitative analysis of venation patterns of Arabidopsis leaves by supervised image analysis. The Plant Journal, 69: 553–563. doi: 10.1111/j.1365-313X.2011.04803.x
- Issue online: 25 JAN 2012
- Version of Record online: 8 NOV 2011
- Accepted manuscript online: 29 SEP 2011 02:27AM EST
- Received 29 June 2011; revised 9 September 2011; accepted 27 September 2011.
Figure S1. Detailed representation of venation segmentation steps.
(a) Segmentation of the venation pattern after grayscale dilation and thresholding of the leaf picture with removed background.
(b) Removal of small structures disconnected from the vascular network (see arrowheads in a).
(c) Thinning of the resulting binary image and removal of small spurs (see arrowheads in b). Panels are zoom-ins of Figure 2g–i.
Figure S2. Branching point and endpoint density during leaf development.
Error bars represent SE (n ≥ 10).
Table S1. Vascular analysis of wild-type plants and six transgenic lines, with contrasting cellular and leaf size phenotypes at 24 DAS.
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