A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots
Article first published online: 2 DEC 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 69, Issue 4, pages 565–576, February 2012
How to Cite
Huang, C.-F., Yamaji, N., Ono, K. and Ma, J. F. (2012), A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots. The Plant Journal, 69: 565–576. doi: 10.1111/j.1365-313X.2011.04824.x
- Issue published online: 6 FEB 2012
- Article first published online: 2 DEC 2011
- Accepted manuscript online: 20 OCT 2011 09:35AM EST
- Received 22 August 2011; revised 10 October 2011; accepted 17 October 2011; published online 2 December 2011.
- outer cell layer;
Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 (Docs1), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map-based cloning using a mutant (c68) defective in the outer cell layers of primary roots. It encodes a leucine-rich repeat receptor-like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti-Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome-wide transcriptional profiles between the wild-type and the knock-out mutant roots using microarray analysis showed that 61 and 41 genes were up- and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.