A genome-wide phylogenetic reconstruction of family 1 UDP-glycosyltransferases revealed the expansion of the family during the adaptation of plants to life on land

Authors

  • Lorenzo Caputi,

    1. Fondazione Edmund Mach, Centro Ricerca e Innovazione, Department of Food Quality and Nutrition, Istituto Agrario San Michele all’Adige–IASMA, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy
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    • These authors equally contributed to this work.

  • Mickael Malnoy,

    1. Fondazione Edmund Mach, Centro Ricerca e Innovazione, Department of Genomics and Biology of Fruit Crop, Istituto Agrario San Michele all’Adige–IASMA, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy
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    • These authors equally contributed to this work.

  • Vadim Goremykin,

    1. Fondazione Edmund Mach, Centro Ricerca e Innovazione, Department of Genomics and Biology of Fruit Crop, Istituto Agrario San Michele all’Adige–IASMA, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy
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  • Svetlana Nikiforova,

    1. Fondazione Edmund Mach, Centro Ricerca e Innovazione, Department of Genomics and Biology of Fruit Crop, Istituto Agrario San Michele all’Adige–IASMA, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy
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  • Stefan Martens

    Corresponding author
    1. Fondazione Edmund Mach, Centro Ricerca e Innovazione, Department of Food Quality and Nutrition, Istituto Agrario San Michele all’Adige–IASMA, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy
      (fax +39 461 615200; e-mail stefan.martens@iasma.it).
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(fax +39 461 615200; e-mail stefan.martens@iasma.it).

Summary

For almost a decade, our knowledge on the organisation of the family 1 UDP-glycosyltransferases (UGTs) has been limited to the model plant A. thaliana. The availability of other plant genomes represents an opportunity to obtain a broader view of the family in terms of evolution and organisation. Family 1 UGTs are known to glycosylate several classes of plant secondary metabolites. A phylogeny reconstruction study was performed to get an insight into the evolution of this multigene family during the adaptation of plants to life on land. The organisation of the UGTs in the different organisms was also investigated. More than 1500 putative UGTs were identified in 12 fully sequenced and assembled plant genomes based on the highly conserved PSPG motif. Analyses by maximum likelihood (ML) method were performed to reconstruct the phylogenetic relationships existing between the sequences. The results of this study clearly show that the UGT family expanded during the transition from algae to vascular plants and that in higher plants the clustering of UGTs into phylogenetic groups appears to be conserved, although gene loss and gene gain events seem to have occurred in certain lineages. Interestingly, two new phylogenetic groups, named O and P, that are not present in A. thaliana were discovered.

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