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Figure S1. Absolute levels of metabolites in Risø16 and Bomi endosperm. Metabolite contents have been analysed by enzyme-coupled spectrophotometric assays and HPLC and are given as absolute values for Risø16 and Bomi (nmol g−1 fresh weight, with the exception of sucrose, glucose, fructose and ammonium which are given in μmol g−1 fresh weight), n = 3–5, statistically different values please refer to Figure 3.

Figure S2. Absolute levels of NAD, NADH, NADP and NADPH in Risø16 and Bomi endosperm. Data are the mean ± SD (= 4). Symbols see Figure S1.

Figure S3. Northern analysis of HvIDH1 and HvIDH2 in Risø16 and Bomi endosperm. The figure shows transcript abundances of HvIDH1 and HvIDH2 in developing endosperm of Risø16 and Bomi between 4 and 20 days after pollination (DAP).

Figure S4. Negative correlations between sugar and amino acid levels in the endosperm of Risø16 and Bomi between 10 and 14 DAP. Values of sugars (sum of sucrose, glucose and fructose) and amino acids have been taken from Figure S1 and S2.

Table S1. Differential gene expression between Risø16 and Bomi as analysed using the AGILENT 44K gene chip, ratios Bomi: Risø16. Values are means of three biological replicates. Boldface, statistically significant according to t-test, P < 0.05.

Table S2. Differential gene expression between Risø16 and Bomi as analysed using the AGILENT 44K gene chip, ratios Bomi: Risø16. The data set from Table S1 was manually reduced and ordered by pathway/functional categories, boldface, statistically significant according to t-test, P < 0.05.

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