These authors contributed equally to this work.
Unraveling the regulatory network of the MADS box transcription factor RIN in fruit ripening
Article first published online: 19 DEC 2011
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 70, Issue 2, pages 243–255, April 2012
How to Cite
Qin, G., Wang, Y., Cao, B., Wang, W. and Tian, S. (2012), Unraveling the regulatory network of the MADS box transcription factor RIN in fruit ripening. The Plant Journal, 70: 243–255. doi: 10.1111/j.1365-313X.2011.04861.x
- Issue published online: 3 APR 2012
- Article first published online: 19 DEC 2011
- Accepted manuscript online: 18 NOV 2011 11:43PM EST
- Received 8 October 2011; accepted 17 November 2011; published online 19 December 2011.
Figure S1. Functional categorization of the differentially expressed proteins identified from the rin mutant tomato fruit. The proteins were classified according to their biological roles and the FunCat annotation scheme (http://mips.gsf.de/proj/funcatDB). The 41 protein spots identified were categorized in 13 classes, i.e. amino acid metabolism, carbohydrate metabolism, lipid metabolism, secondary metabolism, glycolysis, Krebs cycle, photosynthesis/respiration, fermentation, protein modification, intracellular signaling, stress response, detoxification, and cytoskeleton.
Figure S2. Annotated spectra for proteins identified by a single peptide or multiple peptides with each ion scored below the threshold. Spot numbers were consistent with those in 2-DE gel.
Table S1. Identification of the differentially expressed proteins in the rin mutant tomato fruit using ESI-Q-TOF MS/MS
Table S2. Predicted RIN binding motifs within 2000-bp upstream region starting from ATG of each candidate target genes
Table S3. Primers used in the ChIP-qPCR analysis
Table S4. Scores and matched peptides of the identified proteins based on tandem mass spectrometry. Proteins were listed with an order consistent with those in Table S1. The numbers were consistent with those in 2-DE gel. Area highlighted in grey means that these protein spots were identified with only one matching peptide or by multiple peptides with each ion scored below the threshold. The annotated spectra for these proteins were present in Figure S2
Table S5. Primers for qRT-PCR analysis
Table S6. Sequences of the probes used in EMSA
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|TPJ_4861_sm_FigS1.pdf||72K||Supporting info item|
|TPJ_4861_sm_FigS2.pdf||58K||Supporting info item|
|TPJ_4861_sm_SupportingInformation.doc||26K||Supporting info item|
|TPJ_4861_sm_TableS1.pdf||69K||Supporting info item|
|TPJ_4861_sm_TableS2.pdf||17K||Supporting info item|
|TPJ_4861_sm_TableS3.pdf||16K||Supporting info item|
|TPJ_4861_sm_TableS4.xls||169K||Supporting info item|
|TPJ_4861_sm_TableS5.pdf||16K||Supporting info item|
|TPJ_4861_sm_TableS6.pdf||13K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.