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Figure S1. Expression patterns of DTM1. (a) Transcript levels in various organs. (b) Transcript levels in floral organs. Y axis, gene expression relative to rice Ubiquitin (Os03g0234200) transcript level. Results are average of three independent experiments.

Figure S2. Callose deposition in anthers and expression of callose related genes. (a, b) Aniline blue staining of WT (a) and dtm1-1 (b) anthers at Stage 6–8a. Arrows indicate callose wall. Bars: 10 μm. (c) Transcript level of Osg1, a callase gene. (d) Transcript level of OsGSL5, a callose synthase gene. Y axis, gene expression relative to rice Ubiquitin (Os03g0234200) transcript level. Results are average of three independent experiments.

Figure S3. TEM analyses of anthers. (a–d) Cross sections of WT (a, c) and dtm1-1 (b, d) anther walls at early Stage 6 (a, b) and Stage 7 (c, d). (e–h) Magnified images of WT (e, g) and dtm1-1 (f, h) tapetal cells at early Stage 6 (e, f) and Stage 7 (g, h). (i–l) Magnified images of WT (i, k) and dtm1-1 (j, l) PMC at early Stage 6 (i, j) and Stage 7 (k, l). Arrowheads indicate undeveloped and unusual cellular organs. En, endothecial cell; Ep, epidermal cell; lb, lipid body; M, mitochondrion; Ml, middle layer; n, nucleus; p, plastid; PT, peritapetal region; T, tapetum cell. Bars: 1 μm (a–h) or 5 μm (i–l).

Figure S4. TEM analyses of 3rd leaf from WT and dtm1. (a) Mesophyll cell of WT. (b) Magnified image of solid box in a showing concentrated circular form of ER. (c) Magnified image of dashed box in a showing rough ER. (d) Mesophyll cell of dtm1. (e) Magnified image of solid box in d showing concentrated circular form of ER. (f) Magnified image of dashed box in d showing rough ER. Ch, chloroplast; Nu, nucleus. Bars: 5 μm (a, d), 1 μm (b, e), or 0.5 μm (c, f).

Figure S5. Chromosome spreads of WT male meiocytes. (a) diakinesis. (b) metaphase I. (c) anaphase I. (d) telophase I. Bars: 10 μm.

Table S1. Cross pollination test.

Table S2. Primers used for real-time PCR.

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