HIGH-RESOLUTION MEASUREMENTS IN PLANT BIOLOGY
Lipidomics in tissues, cells and subcellular compartments
Article first published online: 27 MAR 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Special Issue: High-resolution Measurements in Plant Biology
Volume 70, Issue 1, pages 69–80, April 2012
How to Cite
Horn, P. J. and Chapman, K. D. (2012), Lipidomics in tissues, cells and subcellular compartments. The Plant Journal, 70: 69–80. doi: 10.1111/j.1365-313X.2011.04868.x
- Issue published online: 27 MAR 2012
- Article first published online: 27 MAR 2012
- Accepted manuscript online: 24 NOV 2011 11:16AM EST
- Received 14 October 2011; revised 15 November 2011; accepted 22 November 2011.
- mass spectrometry;
- lipid droplets
Mass spectrometry (MS) advances in recent years have revolutionized the biochemical analysis of lipids in plants, and made possible new theories about the structural diversity and functional complexity of lipids in plant cells. Approaches have been developed to profile the lipidome of plants with increasing chemical and spatial resolution. Here we highlight a variety of methods for lipidomics analysis at the tissue, cellular and subcellular levels. These procedures allow the simultaneous identification and quantification of hundreds of lipids species in tissue extracts by direct-infusion MS, localization of lipids in tissues and cells by laser desorption/ionization MS, and even profiling of lipids in individual subcellular compartments by direct-organelle MS. Applications of these approaches to achieve improved understanding of plant lipid metabolism, compartmentation and function are discussed.