These authors contributed equally to this work.
H3K36 methylation is critical for brassinosteroid-regulated plant growth and development in rice
Article first published online: 5 JAN 2012
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 70, Issue 2, pages 340–347, April 2012
How to Cite
Sui, P., Jin, J., Ye, S., Mu, C., Gao, J., Feng, H., Shen, W.-H., Yu, Y. and Dong, A. (2012), H3K36 methylation is critical for brassinosteroid-regulated plant growth and development in rice. The Plant Journal, 70: 340–347. doi: 10.1111/j.1365-313X.2011.04873.x
- Issue published online: 3 APR 2012
- Article first published online: 5 JAN 2012
- Accepted manuscript online: 5 DEC 2011 04:50AM EST
- Received 29 September 2011; revised 23 November 2011; accepted 1 December 2011; published online 5 January 2012.
- histone methylation;
- SET domain;
Methylation of histone lysine residues plays an essential role in epigenetic regulation of gene expression in eukaryotes. Enzymes involved in establishment of the repressive H3K9 and H3K27 methylation marks have been previously characterized, but the deposition and function of H3K4 and H3K36 methylation remain uncharacterized in rice. Here, we report that rice SDG725 encodes a H3K36 methyltransferase, and its down-regulation causes wide-ranging defects, including dwarfism, shortened internodes, erect leaves and small seeds. These defects resemble the phenotypes previously described for some brassinosteroid-knockdown mutants. Consistently, transcriptome analyses revealed that SDG725 depletion results in down-regulation by more than two-fold of over 1000 genes, including D11, BRI1 and BU1, which are known to be involved in brassinosteroid biosynthesis or signaling pathways. Chromatin immunoprecipitation analyses showed that levels of H3K36me2/3 are reduced in chromatin at some regions of these brassinosteroid-related genes in SDG725 knockdown plants, and that SDG725 protein is able to directly bind to these target genes. Taken together, our data indicate that SDG725-mediated H3K36 methylation modulates brassinosteroid-related gene expression, playing an important role in rice plant growth and development.