Present address: AIDS Reference Laboratory, Ghent University, De Pintelaan 185, 9000 Gent, Belgium.
Nodule numbers are governed by interaction between CLE peptides and cytokinin signaling
Article first published online: 18 JAN 2012
© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd
The Plant Journal
Volume 70, Issue 3, pages 367–376, May 2012
How to Cite
Mortier, V., De Wever, E., Vuylsteke, M., Holsters, M. and Goormachtig, S. (2012), Nodule numbers are governed by interaction between CLE peptides and cytokinin signaling. The Plant Journal, 70: 367–376. doi: 10.1111/j.1365-313X.2011.04881.x
- Issue published online: 10 APR 2012
- Article first published online: 18 JAN 2012
- Accepted manuscript online: 13 DEC 2011 11:20AM EST
- Received 8 November 2011; accepted 6 December 2011; published online 18 January 2012.
Figure S1. Effect of MtCLE12 knock-down on nodule number. (a) qRT-PCR analysis of MtCLE12 expression in cDNA samples of transgenic roots expressing either 35S:GUS (control) or a MtCLE12 hairpin construct (RNAi MtCLE12). (b) Nodule number at 21 dpi of the same lines as shown in (a). Arrows indicate lines with the lowest level of MtCLE12 expression.
Table S1. Expression analysis of MtCRE1, NIN, ERN1 and NSP2 in transgenic roots ectopically expressing 35S:MtCLE12, 35S:MtCLE13 or 35S:GUS. Experiments were repeated four times and the relative expression levels (average ± SD) compared to the expression in 35S:GUS roots are presented.
Table S2. Primers used for the analysis.
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