Present Address: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg August University Göttingen, Julia Lermontowa Weg 3, 37077 Göttingen, Germany.
Putative members of the Arabidopsis Nup107-160 nuclear pore sub-complex contribute to pathogen defense
Article first published online: 6 MAR 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 70, Issue 5, pages 796–808, June 2012
How to Cite
Wiermer, M., Cheng, Y. T., Imkampe, J., Li, M., Wang, D., Lipka, V. and Li, X. (2012), Putative members of the Arabidopsis Nup107-160 nuclear pore sub-complex contribute to pathogen defense. The Plant Journal, 70: 796–808. doi: 10.1111/j.1365-313X.2012.04928.x
- Issue published online: 22 MAY 2012
- Article first published online: 6 MAR 2012
- Accepted manuscript online: 31 JAN 2012 02:47AM EST
- Received 22 December 2011; accepted 24 January 2012; published online 6 March 2012.
- plant immunity;
- Nup107-160 complex;
- mRNA export;
- nucleocytoplasmic trafficking;
In eukaryotic cells, transduction of external stimuli into the nucleus to induce transcription and export of mRNAs for translation in the cytoplasm is mediated by nuclear pore complexes (NPCs) composed of nucleoporin proteins (Nups). We previously reported that Arabidopsis MOS3, encoding the homolog of vertebrate Nup96, is required for plant immunity and constitutive resistance mediated by the de-regulated Toll interleukin 1 receptor/nucleotide-binding/leucine-rich repeat (TNL)-type R gene snc1. In vertebrates, Nup96 is a component of the conserved Nup107-160 nuclear pore sub-complex, and implicated in immunity-related mRNA export. Here, we used a reverse genetics approach to examine the requirement for additional subunits of the predicted Arabidopsis Nup107-160 complex in plant immunity. We show that, among eight putative complex members, beside MOS3, only plants with defects in Nup160 or Seh1 are impaired in basal resistance. Constitutive resistance in the snc1 mutant and immunity mediated by TNL-type R genes also depend on functional Nup160 and have a partial requirement for Seh1. Conversely, resistance conferred by coiled coil-type immune receptors operates largely independently of both genes, demonstrating specific contributions to plant defense signaling. Our functional analysis further revealed that defects in nup160 and seh1 result in nuclear accumulation of poly(A) mRNA, and, in the case of nup160, considerable depletion of EDS1, a key positive regulator of basal and TNL-triggered resistance. These findings suggest that Nup160 is required for nuclear mRNA export and full expression of EDS1-conditioned resistance pathways in Arabidopsis.