Role of pectin methylesterases in cellular calcium distribution and blossom-end rot development in tomato fruit
Article first published online: 28 JUN 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 71, Issue 5, pages 824–835, September 2012
How to Cite
de Freitas, S. T., Handa, A. K., Wu, Q., Park, S. and Mitcham, E. J. (2012), Role of pectin methylesterases in cellular calcium distribution and blossom-end rot development in tomato fruit. The Plant Journal, 71: 824–835. doi: 10.1111/j.1365-313X.2012.05034.x
- Issue published online: 24 AUG 2012
- Article first published online: 28 JUN 2012
- Accepted manuscript online: 8 MAY 2012 01:21AM EST
- Received 8 February 2011; revised 19 April 2012; accepted 23 April 2012; Published online 28 June 2012.
Figure S1. Negative confocal microscopy control images of wild type (A, B, C) and PME-silenced (D, E, F) fruit pericarp cells at 45 DAP. Green channel (A and D). White channel (B and E). Overlapping of Fluo-4 fluorescence and white channel images (C and F).
Figure S2. Negative electron microscopy pictures showing pericarp cell of wild type (A) and PME-silenced (B) tomato pericarp at 45 DAP treated with EGTA to remove Ca2+ from the potassium antimonate-Ca precipitates (black spots). Arrows pointing to structures similar to the antimonate-Ca precipitates observed inside the vacuole of cells without EGTA treatment. V = vacuole, CW = cell wall, Cyt = cytosol.
Table S1. Primer sequences used for expression analysis of PME genes.
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