These authors contributed equally to this work.
A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis
Article first published online: 9 JUL 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 71, Issue 6, pages 948–961, September 2012
How to Cite
Short, E. F., North, K. A., Roberts, M. R., Hetherington, A. M., Shirras, A. D. and McAinsh, M. R. (2012), A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis. The Plant Journal, 71: 948–961. doi: 10.1111/j.1365-313X.2012.05043.x
- Issue published online: 6 SEP 2012
- Article first published online: 9 JUL 2012
- Accepted manuscript online: 5 MAY 2012 11:10AM EST
- Received 24 January 2012; revised 25 April 2012; accepted 1 May 2012; published online 9 July 2012.
- Arabidopsis thaliana;
- signal transduction;
- gene expression;
- reactive oxygen species
Changes in gene expression form a key component of the molecular mechanisms by which plants adapt and respond to environmental stresses. There is compelling evidence for the role of stimulus-specific Ca2+ signatures in plant stress responses. However, our understanding of how they orchestrate the differential expression of stress-induced genes remains fragmentary. We have undertaken a global study of changes in the Arabidopsis transcriptome induced by the pollutant ozone in order to establish a robust transcriptional response against which to test the ability of Ca2+ signatures to encode stimulus-specific transcriptional information. We show that the expression of a set of co-regulated ozone-induced genes is Ca2+-dependent and that abolition of the ozone-induced Ca2+ signature inhibits the induction of these genes by ozone. No induction of this set of ozone-regulated genes was observed in response to H2O2, one of the reactive oxygen species (ROS) generated by ozone, or cold stress, which also generates ROS, both of which stimulate changes in [Ca2+]cyt. These data establish unequivocally that the Ca2+-dependent changes in gene expression observed in response to ozone are not simply a consequence of an ROS-induced increase in [Ca2+]cytper se. The magnitude and temporal dynamics of the ozone, H2O2, and cold Ca2+ signatures all differ markedly. This finding is consistent with the hypothesis that stimulus-specific transcriptional information can be encoded in the spatiotemporal dynamics of complex Ca2+ signals in plants.