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Figure S1. Acute ozone stress causes visible damage but does not impair growth. Seven day-old Arabidopsis seedling were exposed to 300 ppb (open bars) or 500 ppb (hatched bars) ozone, or ozone-free air (solid bars, 300 ppb ozone control; crosshatched bars, 500 ppb ozone control). Ozone-induced damage was assessed 24 h and 48 h after the end of the treatment: (a) Visible damage, (b) Relative ion leakage. Values are means ± SEM (n = 3).

Figure S2. Expression of ozone-induced genes used in this study in publically available microarray data sets. Heatmap of expression signals in selected public microarray data sets in the Genevestigator database (www.genevestigator.com/gv/) for the 5 genes used to assay ozone responses.  The eight experiments shown include, from top to bottom; response to high light stress-induced H2O2 in WT and a catalyse-deficient mutant (Vanderauwera et al (2005) Plant Physiol. 139, 806-821), oxidative stress response (methyl viologen) within the AtGenExpress dataset (Kilian et al (2007) Plant J. 50, 347-363), response to H2O2 application (Davetlova et al (2005) Plant Physiol. 139, 847-856), response to Pseudomonas syringae infection (Bartsch et al (2006) Plant Cell 18, 1038-1051), expression in the singlet oxygen-producing flu mutant (Laloi et al (2007) Proc. Natl. Acad. Sci. USA 104, 672-677), response to Blumeria graminis infection (Jensen et al (2008) Plant J. 56, 867-880), response to superoxide generated by methyl viologen (Scarpeci et al (2008) Plant Mol. Biol. 66, 361-378), response to Phytophthora parasitica infection (unpublished; Genevestigator experiment ID AT-00425).

Table S1. Ca2+ signalling (MapMan bin 30.3) is amongst the most significantly over-represented functional groups of genes which are transcriptionally altered by ozone treatment. This group includes 209 Affymetrix probes, of which 52 (shown here) are up- ([UPWARDS ARROW]) and 6 down- ([DOWNWARDS ARROW]) regulated (mean fold-change >2) by ozone.

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