These authors contributed equally to this work.
A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis
Article first published online: 9 JUL 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 71, Issue 6, pages 948–961, September 2012
How to Cite
Short, E. F., North, K. A., Roberts, M. R., Hetherington, A. M., Shirras, A. D. and McAinsh, M. R. (2012), A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis. The Plant Journal, 71: 948–961. doi: 10.1111/j.1365-313X.2012.05043.x
- Issue published online: 6 SEP 2012
- Article first published online: 9 JUL 2012
- Accepted manuscript online: 5 MAY 2012 11:10AM EST
- Received 24 January 2012; revised 25 April 2012; accepted 1 May 2012; published online 9 July 2012.
Figure S1. Acute ozone stress causes visible damage but does not impair growth. Seven day-old Arabidopsis seedling were exposed to 300 ppb (open bars) or 500 ppb (hatched bars) ozone, or ozone-free air (solid bars, 300 ppb ozone control; crosshatched bars, 500 ppb ozone control). Ozone-induced damage was assessed 24 h and 48 h after the end of the treatment: (a) Visible damage, (b) Relative ion leakage. Values are means ± SEM (n = 3).
Figure S2. Expression of ozone-induced genes used in this study in publically available microarray data sets. Heatmap of expression signals in selected public microarray data sets in the Genevestigator database (www.genevestigator.com/gv/) for the 5 genes used to assay ozone responses. The eight experiments shown include, from top to bottom; response to high light stress-induced H2O2 in WT and a catalyse-deficient mutant (Vanderauwera et al (2005) Plant Physiol. 139, 806-821), oxidative stress response (methyl viologen) within the AtGenExpress dataset (Kilian et al (2007) Plant J. 50, 347-363), response to H2O2 application (Davetlova et al (2005) Plant Physiol. 139, 847-856), response to Pseudomonas syringae infection (Bartsch et al (2006) Plant Cell 18, 1038-1051), expression in the singlet oxygen-producing flu mutant (Laloi et al (2007) Proc. Natl. Acad. Sci. USA 104, 672-677), response to Blumeria graminis infection (Jensen et al (2008) Plant J. 56, 867-880), response to superoxide generated by methyl viologen (Scarpeci et al (2008) Plant Mol. Biol. 66, 361-378), response to Phytophthora parasitica infection (unpublished; Genevestigator experiment ID AT-00425).
Table S1. Ca2+ signalling (MapMan bin 30.3) is amongst the most significantly over-represented functional groups of genes which are transcriptionally altered by ozone treatment. This group includes 209 Affymetrix probes, of which 52 (shown here) are up- () and 6 down- () regulated (mean fold-change >2) by ozone.
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