CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signalling pathway

Authors

  • Louis-Jérôme Leba,

    1. Université de Toulouse, Université de Toulouse (UPS), UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP 42617, F-31326 Castanet-Tolosan Cedex, France
    2. Centre National de la Recherche Scientifique, UMR 5546, BP 42617, F-31326 Castanet-Tolosan Cedex, France
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    • These authors contributed equally to this work.

  • Cécilia Cheval,

    1. Université de Toulouse, Université de Toulouse (UPS), UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP 42617, F-31326 Castanet-Tolosan Cedex, France
    2. Centre National de la Recherche Scientifique, UMR 5546, BP 42617, F-31326 Castanet-Tolosan Cedex, France
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    • These authors contributed equally to this work.

  • Inmaculada Ortiz-Martín,

    1. Instituto de Hortofruticultura Subtropical y Mediterranea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Celular, Genética y Fisiología, Campus de Teatinos, Málaga 29071, Spain
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  • Benoit Ranty,

    1. Université de Toulouse, Université de Toulouse (UPS), UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP 42617, F-31326 Castanet-Tolosan Cedex, France
    2. Centre National de la Recherche Scientifique, UMR 5546, BP 42617, F-31326 Castanet-Tolosan Cedex, France
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  • Carmen R. Beuzón,

    1. Instituto de Hortofruticultura Subtropical y Mediterranea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Celular, Genética y Fisiología, Campus de Teatinos, Málaga 29071, Spain
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  • Jean-Philippe Galaud,

    1. Université de Toulouse, Université de Toulouse (UPS), UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP 42617, F-31326 Castanet-Tolosan Cedex, France
    2. Centre National de la Recherche Scientifique, UMR 5546, BP 42617, F-31326 Castanet-Tolosan Cedex, France
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  • Didier Aldon

    Corresponding author
    1. Université de Toulouse, Université de Toulouse (UPS), UMR 5546, Laboratoire de Recherche en Sciences Végétales, BP 42617, F-31326 Castanet-Tolosan Cedex, France
    2. Centre National de la Recherche Scientifique, UMR 5546, BP 42617, F-31326 Castanet-Tolosan Cedex, France
      (e-mail aldon@lrsv.ups-tlse.fr).
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(e-mail aldon@lrsv.ups-tlse.fr).

Summary

Many stimuli such as hormones and elicitors induce changes in intracellular calcium levels to integrate information and activate appropriate responses. The Ca2+ signals are perceived by various Ca2+ sensors, and calmodulin (CaM) is one of the best characterized in eukaryotes. Calmodulin-like (CML) proteins extend the Ca2+ toolkit in plants; they share sequence similarity with the ubiquitous and highly conserved CaM but their roles at physiological and molecular levels are largely unknown. Knowledge of the contribution of Ca2+ decoding proteins to plant immunity is emerging, and we report here data on Arabidopsis thaliana CML9, whose expression is rapidly induced by phytopathogenic bacteria, flagellin and salicylic acid. Using a reverse genetic approach, we present evidence that CML9 is involved in plant defence by modulating responses to bacterial strains of Pseudomonas syringae. Compared to wild-type plants, the later responses normally observed upon flagellin application are altered in knockout mutants and over-expressing transgenic lines. Collectively, using PAMP treatment and P. syringae strains, we have established that CML9 participates in plant innate immunity.

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