CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites
Article first published online: 23 AUG 2012
© 2012 Institut National de la Recherche Agronomic. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 2, pages 185–198, October 2012
How to Cite
de Almeida Engler, J., Kyndt, T., Vieira, P., Van Cappelle, E., Boudolf, V., Sanchez, V., Escobar, C., De Veylder, L., Engler, G., Abad, P. and Gheysen, G. (2012), CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites. The Plant Journal, 72: 185–198. doi: 10.1111/j.1365-313X.2012.05054.x
- Issue published online: 16 OCT 2012
- Article first published online: 23 AUG 2012
- Accepted manuscript online: 28 MAY 2012 08:50AM EST
- Received 9 November 2011; revised 13 April 2012; accepted 4 May 2012; published online 23 August 2012.
- cell cycle;
- root-knot nematode;
- cyst nematode
The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.