CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites

Authors

  • Janice de Almeida Engler,

    Corresponding author
    1. Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France
      (e-mails janice.almeida-engler@sophia.inra.fr or godelieve.gheysen@ugent.be).
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  • Tina Kyndt,

    1. Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium
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  • Paulo Vieira,

    1. Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France
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  • Elke Van Cappelle,

    1. Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium
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  • Veronique Boudolf,

    1. Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
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  • Vanesa Sanchez,

    1. Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France
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  • Carolina Escobar,

    1. Unidad de Genómica, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain
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  • Lieven De Veylder,

    1. Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium
    2. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
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  • Gilbert Engler,

    1. Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France
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  • Pierre Abad,

    1. Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France
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  • Godelieve Gheysen

    Corresponding author
    1. Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium
      (e-mails janice.almeida-engler@sophia.inra.fr or godelieve.gheysen@ugent.be).
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(e-mails janice.almeida-engler@sophia.inra.fr or godelieve.gheysen@ugent.be).

Summary

The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.

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