The role of cytokinin during Arabidopsis gynoecia and fruit morphogenesis and patterning
Article first published online: 7 AUG 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 2, pages 222–234, October 2012
How to Cite
Marsch-Martínez, N., Ramos-Cruz, D., Irepan Reyes-Olalde, J., Lozano-Sotomayor, P., Zúñiga-Mayo, V. M. and de Folter, S. (2012), The role of cytokinin during Arabidopsis gynoecia and fruit morphogenesis and patterning. The Plant Journal, 72: 222–234. doi: 10.1111/j.1365-313X.2012.05062.x
- Issue published online: 16 OCT 2012
- Article first published online: 7 AUG 2012
- Accepted manuscript online: 28 MAY 2012 10:05AM EST
- Received 9 September 2011; revised 15 May 2012; accepted 23 May 2012; published online 7 August 2012.
- Arabidopsis gynoecium and fruit development;
- valve margin;
- cell proliferation;
Cytokinins have many essential roles in embryonic and post-embryonic growth and development, but their role in fruit morphogenesis is currently not really known. Moreover, information about the spatio-temporal localization pattern of cytokinin signaling in gynoecia and fruits is lacking. Therefore, the synthetic reporter line TCS::GFP was used to visualize cytokinin signaling during gynoecium and fruit development. Fluorescence was detected at medial regions of developing gynoecia, and, unexpectedly, at the valve margin in developing fruits, and was severely altered in mutants that lack or ectopically acquire valve margin identity. Comparison to developing gynoecia and fruits in a DR5rev::GFP line showed that the transcriptional responses to cytokinin and auxin are frequently present in complementary patterns. Moreover, cytokinin treatments in early gynoecia produced conspicuous changes, and treatment of valve margin mutant fruits restored this tissue. The results suggest that the phytohormone cytokinin is important in gynoecium and fruit patterning and morphogenesis, playing at least two roles: an early proliferation-inducing role at the medial tissues of the developing gynoecia, and a late role in fruit patterning and morphogenesis at the valve margin of developing fruits.